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Related Experiment Video

Updated: Apr 15, 2026

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Published on: June 9, 2021

Integrated Optimized HPLC-MS/MS Profiling and GWAS Uncover Candidate Genes for Folate Content in Cucumber Fruits.

Qi Zhou^1,2,3,4, Jiantao Guan¹, Han Miao¹

¹State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Journal of Agricultural and Food Chemistry

|April 13, 2026

Summary

Researchers identified key genes and genetic markers for increasing folate levels in cucumbers. This breakthrough aids in developing healthier cucumber varieties rich in essential B vitamins for improved nutrition.

Keywords:

GWAS HPLC-MS/MS cucumber folate quantification

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Last Updated: Apr 15, 2026

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

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Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Area of Science:

Agricultural Science
Nutritional Biochemistry
Plant Genetics

Background:

Folate (vitamin B) is vital for human health.
Cucumbers are a common raw vegetable and a potential dietary folate source.
Breeding high-folate cucumbers is hindered by poor understanding of folate content and genetic control.

Purpose of the Study:

To develop a precise method for quantifying folate derivatives in cucumbers.
To investigate folate variation within a cucumber germplasm collection.
To identify genetic factors controlling folate accumulation in cucumbers.

Main Methods:

Optimized High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) for 12 folate derivatives.
Folate analysis in cucumber fruits across two growing seasons.
Genome-Wide Association Study (GWAS) to pinpoint genetic loci.

Main Results:

5-methyl-tetrahydrofolate (5-M-THF) was the predominant folate derivative.
Cucumber varieties from South China showed the highest folate content.
GWAS identified 35 significant loci, including 8 pleiotropic loci, associated with folate levels.
Four candidate genes (CsaV3_3G033660, CsaV3_3G033670, CsaV3_1G029540, CsaV3_1G029550) were identified.

Conclusions:

This study establishes an accurate method for folate quantification in high-moisture crops.
Genetic insights and molecular markers are provided for breeding folate-enhanced cucumbers.
Findings facilitate the development of cucumbers with improved nutritional value.