Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanical and Chemical Digestion in the Small Intestine01:30

Mechanical and Chemical Digestion in the Small Intestine

4.8K
The small intestine plays a crucial role in our digestive system, performing both mechanical and chemical digestion.
Mechanical digestion in the small intestine involves movements such as segmentations and migrating motility complexes (MMCs), primarily controlled by the myenteric plexus. Segmentations are localized contractions occurring in areas of the intestine distended by chyme—a mixture of partially digested food. These contractions mix chyme with digestive juices, facilitating...
4.8K
Designing Growth Media for Bioreactors01:30

Designing Growth Media for Bioreactors

67
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
67
Bioavailability Enhancement: Drug Stability Enhancement and GI Retention01:05

Bioavailability Enhancement: Drug Stability Enhancement and GI Retention

314
Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
314
Upstream Processing01:27

Upstream Processing

87
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
87
Probiotics01:22

Probiotics

231
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
231

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Promise of 3D Biomaterial Bioprinting for Wound-Healing and Skin Tissue Restoration.

Life (Basel, Switzerland)·2026
Same author

Evolution of the Chick Embryo Chorioallantoic Membrane Proteome during Early Development.

ACS omega·2026
Same author

An in-depth evaluation of fucoidans from five brown seaweeds for wound healing and cytoprotection: analyses of bioactivities and cellular mechanisms.

International journal of biological macromolecules·2025
Same author

<i>In vitro</i> modeling of the female gut microbiome: effects of sex hormones and psychotropic drugs.

Microbiology spectrum·2025
Same author

Structural characterization and in vitro bioactivity of Furcellaria lumbricalis polysaccharides: Implications for skin, immune, and gut health applications.

International journal of biological macromolecules·2025
Same author

Serotonergic and immunomodulatory properties of the psychobiotic candidate <i>Bacteroides finegoldii</i> UO.H1052 and its extracellular vesicles.

Applied and environmental microbiology·2025
Same journal

RETRACTED: Atta et al. Effect of Montmorillonite Nanogel Composite Fillers on the Protection Performance of Epoxy Coatings on Steel Pipelines. <i>Molecules</i> 2017, <i>22</i>, 905.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Chen et al. Chemical Composition of <i>Litsea pungens</i> Essential Oil and Its Potential Antioxidant and Antimicrobial Activities. <i>Molecules</i> 2023, <i>28</i>, 6835.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Ruan et al. Comparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of <i>Cistanche</i>. <i>Molecules</i> 2025, <i>30</i>, 4754.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Li et al. Gastrodin Ameliorates Cognitive Dysfunction in Vascular Dementia Rats by Suppressing Ferroptosis via the Regulation of the Nrf2/Keap1-GPx4 Signaling Pathway. <i>Molecules</i> 2022, <i>27</i>, 6311.

Molecules (Basel, Switzerland)·2026
Same journal

Correction: Zueva et al. Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P-S Bonded Organophosphorus as Monitored by Spectrofluorimetry. <i>Molecules</i> 2020, <i>25</i>, 1371.

Molecules (Basel, Switzerland)·2026
Same journal

1,4-Diazatriphenylene and Its Hetero-Fused Analogs: Synthesis and Applications.

Molecules (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Apr 15, 2026

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers
09:44

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers

Published on: July 7, 2023

5.3K

Scalable Jet-O-Mized Eggshell Membrane Processing for Bioactives Promoting Gut Health.

Manar Younes1, Tamer A E Ahmed1, Riadh Hammami2

  • 1Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

Molecules (Basel, Switzerland)
|April 14, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to create bioactive eggshell membrane (ESM) formulations. These novel ESM derivatives show promising antibacterial, antioxidant, and anti-inflammatory effects for promoting gut health.

Keywords:
alkaline hydrolysisanti-inflammatoryantibacterialantioxidanteggshell membranegut permeabilityproteins

More Related Videos

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
11:53

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Published on: March 10, 2016

9.3K
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
06:12

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

5.6K

Related Experiment Videos

Last Updated: Apr 15, 2026

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers
09:44

Author Spotlight: Advancing Cell Therapy Manufacturing with Dissolvable Microcarriers

Published on: July 7, 2023

5.3K
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
11:53

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Published on: March 10, 2016

9.3K
Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
06:12

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Published on: January 27, 2021

5.6K

Area of Science:

  • Biomaterials Science
  • Nutraceuticals
  • Gastroenterology

Background:

  • Eggshell membrane (ESM) is an underutilized biomaterial.
  • Developing scalable and effective methods for ESM bioactive extraction is needed for health applications.

Purpose of the Study:

  • To develop a simplified, scalable two-step process for generating bioactive ESM formulations.
  • To evaluate the gut health applications of these novel ESM derivatives.

Main Methods:

  • Jet-O-mizer ultra-fine milling of ESM followed by KOH-mediated hydrolysis.
  • Separation of soluble protein fraction (SJ) and simulated gastrointestinal digestion.
  • Assessment of antioxidant capacity, antibacterial activity against E. coli, anti-inflammatory effects on RAW macrophages, and Caco-2 cell permeability.

Main Results:

  • A 15-fold increase in antioxidant capacity compared to non-hydrolyzed JEM.
  • SJ inhibited E. coli growth by 50% and reduced NO production in LPS-induced macrophages by 80%.
  • Proteomics identified key anti-inflammatory and antimicrobial proteins; no significant impact on intestinal barrier integrity.

Conclusions:

  • Novel, safe, and highly bioavailable ESM derivatives with multifunctional bioactivities were successfully generated.
  • These ESM derivatives hold promise as dietary supplements for promoting gut health.