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Related Experiment Video

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Advances of Digital Detection for Foodborne Pathogens.

Ruonan He1, Diming Hua2, Wenwen Wu3

  • 1College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.

Foods (Basel, Switzerland)
|April 14, 2026
PubMed
Summary

Digital detection offers ultra-sensitive methods for identifying foodborne pathogens, crucial for robust food safety. Advances in platforms and AI promise intelligent, data-driven surveillance strategies for enhanced monitoring.

Keywords:
biosensingdigital detectionfood safetyfoodborne pathogennucleic acid

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Area of Science:

  • Food safety analysis
  • Microbiology
  • Analytical chemistry

Background:

  • Stringent regulations demand highly sensitive methods for detecting foodborne pathogens.
  • Digital detection provides absolute quantification and matrix tolerance for reliable food safety monitoring.

Purpose of the Study:

  • To review recent advancements in digital detection technologies for foodborne pathogens.
  • To highlight applications in viable bacteria quantification, antimicrobial resistance analysis, and multiplex detection.
  • To discuss emerging trends like partition-free detection and AI-assisted analysis.

Main Methods:

  • Review of nucleic acid amplification-based platforms (droplet digital PCR, digital isothermal amplification).
  • Exploration of preamplification-free approaches utilizing enzyme-mediated signal conversion, nanomaterials, and microfluidics.
  • Analysis of AI-assisted and partition-free digital detection trends.

Main Results:

  • Digital detection technologies enable highly specific and accurate identification of foodborne pathogens.
  • Current methods show promise for quantifying viable bacteria and analyzing antimicrobial resistance.
  • Emerging trends focus on enhancing efficiency and integrating artificial intelligence.

Conclusions:

  • Digital detection is a powerful tool for advancing food safety surveillance.
  • Continued innovation in digital platforms and AI will drive the development of intelligent, data-driven strategies.
  • These advancements are key to meeting regulatory demands and ensuring public health.