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Updated: Apr 15, 2026

Cheek Injection Model for Simultaneous Measurement of Pain and Itch-related Behaviors
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CLARIX FLO Inhibits DRG Adhesion-Induced Neuropathic Pain Through the CD44-TRPV1 Signaling Pathway

Chia-Chi Kung1,2, Shih-Ping Dai2, Chao-Chiang Tu1,3

  • 1School of Medicine, College of Medicine, Fu Jen Catholic University, No. 510 Zhongzheng Rd., Xinzhuang Dist., New Taipei 24205, Taiwan.

International Journal of Molecular Sciences
|April 14, 2026
PubMed

Insights

CLARIX FLO effectively treats neuropathic pain caused by DRG adhesion. This human amniotic membrane product reduces inflammation and pain by targeting CD44 and TRPV1 pathways.

Area of Science:

  • Neuroscience
  • Regenerative Medicine
  • Pain Research

Background:

  • Dorsal root ganglion (DRG) adhesion is a significant factor in failed back surgery syndrome and neuropathic pain.
  • Adhesions often result from spinal surgery, disc material leakage, or inflammation, necessitating better models for study.

Purpose of the Study:

  • To investigate the therapeutic efficacy of CLARIX FLO, a novel human amniotic membrane and umbilical cord tissue product, in an animal model of DRG adhesion-induced neuropathic pain.
  • To elucidate the underlying mechanisms of CLARIX FLO's action in alleviating pain and inflammation.

Main Methods:

  • Development of a reliable animal model for DRG adhesion-induced neuropathic pain.
  • Administration of CLARIX FLO to the injured DRG and assessment of pain behaviors (mechanical allodynia).
  • Histological analysis of DRG tissue, including outer sheath thickening and inflammatory markers.
  • Investigation of molecular mechanisms involving CD44, TNF-α, and TRPV1.

Main Results:

  • CLARIX FLO significantly attenuated mechanical allodynia and reduced DRG outer sheath thickening.
  • Treatment suppressed the inflammatory microenvironment and decreased hypersensitivity of isolectin B4-positive neurons.
  • CLARIX FLO inhibited TNF-α and TRPV1 overexpression, with CD44 identified as a key mediator.

Conclusions:

  • CLARIX FLO demonstrates significant analgesic and anti-inflammatory effects in a DRG adhesion model.
  • The therapeutic mechanism involves modulation of the CD44-TRPV1 pathway, offering a potential treatment for neuropathic pain associated with DRG adhesion.
Keywords:
CLARIX FLOanti-inflammationdorsal root ganglionneuropathic painoxidative regenerative cellulose

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