Influence of Rapana venosa Protein Hydrolysate on the Mechanical and Optical Performance of Polysaccharide-Based...

In this study, a multicomponent composite film system based on alginate, chitosan, κ-carrageenan, agar, and Rapana venosa protein hydrolysate (RVPH) was developed, and the effect of RVPH incorporation (0-1.5%) on molecular interactions, microstructure, and functional performance was evaluated using FTIR, SEM, mechanical testing, optical analysis, and water-related measurements. FTIR results indicated that RVPH interacted with the polysaccharide matrix mainly through hydrogen bonding and ionic interactions without causing chemical degradation. SEM analysis revealed concentration-dependent microstructural changes, with smoother morphologies at low RVPH levels and increased roughness and heterogeneity at higher concentrations. These structural differences were reflected in the functional properties. All films exhibited high swelling and water solubility. Optical properties were significantly affected by RVPH. Mechanical properties exhibited a non-linear response, with numerical variations observed but no statistically significant differences (p > 0.05). The EDAS and SWARA methods were employed to determine the optimal incorporation level of RVPH in the film formulations. Among the RVPH-containing films, the formulation incorporating 1% RVPH was identified as the most suitable alternative.