Functional Trait Divergence Underlies the Spatial Trade-Off Between Water and Nitrogen Use Efficiencies in Northern...

The coupling of water and nitrogen (N) availability critically constrains alpine plant growth and ecosystem productivity, yet the mechanistic links between plant functional traits and resource use efficiencies (rain use efficiency, RUE; nitrogen use efficiency, NUE) along precipitation gradients remain unclear. This study aimed to test whether coordinated shifts in plant functional traits are associated with spatial variation in RUE and NUE across a precipitation gradient on the Changtang Plateau. Here, combining transect surveys with N-addition experiments on the Changtang Plateau, we measured biomass and leaf/root functional traits on four typical grasslands and analyzed the spatial variations in RUE, NUE, and fertilizer use efficiency (FUE). Our results demonstrated contrasting spatial patterns: with increasing precipitation, soil resource availability, community species richness, and biomass significantly improved, and vegetation shifted from a water-conservative strategy in arid regions to a nutrient-efficient strategy in humid regions. FUE increased with precipitation (p < 0.05), with low-dose nitrogen addition exerting more pronounced effects in humid regions, indicating greater responsiveness to fertilization. This transition in resource use patterns is underpinned by a coordinated divergence in functional traits: as water limitation eases, communities exhibited decreasing specific root length (high specific root length, SRL) coupled with increasing specific leaf area (high specific leaf area, SLA) along the gradient. Our findings demonstrate that functional trait variation is associated with the optimization of resource acquisition across environmental gradients. These results provide a mechanistic basis for adaptive management in climate-sensitive alpine biomes, where differentiated grassland management schemes may enhance ecosystem productivity-water conservation and reduced disturbance in arid regions, with moderate low-dose nitrogen fertilization and species diversity protection in humid regions. Long-term ecosystem responses to such management approaches require further investigation.