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Updated: Apr 15, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Bacterial Fluorescence Signals Predict Dermal Regeneration Template Integration Outcomes.

Carlos Daniel Torres García¹, Erik Hanson-Viana¹, Hecly Lya Vazquez Morales¹

¹Plastic and Reconstructive Surgery Department, Hospital General Dr Rubén Leñero, México.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society

|April 14, 2026

Summary

Intraoperative bacterial fluorescence imaging accurately predicts dermal regeneration template (DRT) success in complex wounds. This advanced imaging technique helps identify bacterial presence, improving wound bed preparation and reducing DRT failure rates.

Keywords:

MolecuLight bacterial infection dermal regeneration template fluorescence imaging intraoperative tools trauma wound

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Last Updated: Apr 15, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

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Cell Membrane Repair Assay Using a Two-photon Laser Microscope

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Published on: January 2, 2018

Area of Science:

Biomedical Engineering
Wound Healing
Surgical Innovation

Background:

Complex wounds with exposed bone or tendon pose reconstructive challenges.
Dermal regeneration template (DRT) use is associated with high failure rates, often due to undetected infections.
Current wound assessment methods fail to reliably detect significant bacterial loads.

Purpose of the Study:

To evaluate intraoperative bacterial fluorescence imaging for predicting DRT outcomes in complex wounds.
To assess the accuracy of fluorescence imaging in detecting bacterial contamination impacting DRT integration.
To compare fluorescence-guided cultures with traditional methods for pathogen detection.

Main Methods:

Prospective observational study of 13 patients with complex wounds undergoing DRT placement.
Blinded intraoperative bacterial fluorescence imaging was performed prior to DRT insertion.
Modified Levine and fluorescence-guided cultures were collected and analyzed.
DRT outcomes were assessed, correlating with fluorescence imaging findings.

Main Results:

Overall DRT complication rate was 63%, with 56% experiencing partial failure despite pre-operative clearance.
DRTs placed on fluorescence-negative areas had an 85.6% success rate.
DRTs placed on fluorescence-positive areas had a significantly lower success rate of 14.4%.
Fluorescence-guided cultures detected pathogens more frequently than traditional methods.

Conclusions:

Intraoperative bacterial fluorescence imaging shows high predictive accuracy for DRT outcomes.
This imaging modality can guide effective wound bed preparation, improving DRT integration.
Fluorescence imaging holds potential as an adjunctive tool for real-time bacterial detection in complex wound management.