Photoactivated Ruthenium-Manganese Dinuclear Complex for Tracking and Two-Pronged Antibacterial Therapy Combining CO...

Area of Science:

• Inorganic Chemistry
• Materials Science
• Biomedical Engineering

Background:

• Antibiotic resistance necessitates novel antibacterial strategies.
• Multimodal synergistic approaches offer enhanced efficacy against bacterial infections.
• Transition metal complexes show potential as therapeutic agents.

Purpose of the Study:

• To develop a visible light-triggered ruthenium-manganese dinuclear complex for multimodal antibacterial therapy.
• To investigate the complex's ability to generate carbon monoxide (CO) and singlet oxygen (¹O₂).
• To evaluate the self-reporting capability of the complex for real-time monitoring.

Main Methods:

• Synthesis and characterization of the ruthenium-manganese dinuclear complex, Ru(mfibpy)Mn.
• Spectroscopic analysis to confirm light-triggered release of CO and generation of ¹O₂.
• In vitro antibacterial assays against Gram-negative and Gram-positive bacteria, including drug-resistant strains and biofilms.
• In vivo studies using a murine-infected wound model.

Main Results:

• Ru(mfibpy)Mn successfully generated CO and ¹O₂ upon visible light irradiation (λmax = 410 nm).
• The complex exhibited a self-reporting luminescence turn-on mechanism for real-time monitoring.
• Synergistic antibacterial effects led to complete bacterial eradication at 1 μM concentration within 10 min.
• Effective disruption of drug-resistant bacterial biofilms and potent therapeutic efficacy in vivo were observed.

Conclusions:

• The developed Ru(mfibpy)Mn complex is a multifunctional molecular tool for combating bacterial infections.
• Visible light-triggered multimodal therapy offers a promising strategy against antibiotic-resistant bacteria.
• The self-reporting feature enables precise control and monitoring of the therapeutic process.