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Updated: Apr 16, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
Immune responses associated with protection from SARS-CoV-2 infection
Arnold Reynaldi1, Wen Shi Lee2, Kevin J Selva2
1Kirby Institute, University of New South Wales, Kensington, New South Wales, Australia.
None:
Conflicting results have emerged regarding immune correlates of protection against SARS-CoV-2. In this secondary analysis of a trial of immediate versus delayed bivalent mRNA booster vaccination, we studied a range of humoral and cellular immune responses 28 days after vaccination and analyzed their association with subsequent symptomatic and asymptomatic SARS-CoV-2 infection. We found neutralizing antibodies and other plasma antibody titers correlated with prevention of SARS-CoV-2 infection. In a multivariate model, only neutralizing antibodies remained associated with protection from infection (p = 0.021). There was no significant correlation observed between B and T cell responses to Spike and protection. Our findings add to evidence of antibody correlates of prevention of SARS-CoV-2 infection. Trial registration: ANZCTR 12622000411741.
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