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Updated: Apr 16, 2026

Chromatin Immunoprecipitation ChIP using Drosophila tissue
Published on: March 23, 2012
DAXX directs dual modes of H3.4-to-H3.3 histone replacement in the male germline
Yu-Han Yeh1, Mengwen Hu1, Kai Otsuka2
1Department of Microbiology and Molecular Genetics, University of California, Davis, Davis, California 95616, USA.
Abstract:
During spermatogenesis, extensive chromatin remodeling and histone replacement reshape the male germline epigenome. Although HIRA mediates transcription-coupled incorporation of histone variant H3.3, we identified DAXX as a key histone chaperone directing genome-wide, transcription-coupled replacement of H3.4 (H3T) with H3.3 on autosomes during male meiosis. Simultaneously, DAXX also directs transcription-independent H3.4-to-H3.3 replacement on the sex chromosomes during meiotic sex chromosome inactivation (MSCI). These distinct, chromosome-specific modes of DAXX-mediated H3.3 deposition are essential for epigenomic integrity in the male germline. Loss of DAXX disrupts this process, resulting in widespread transcriptional dysregulation in haploid round spermatids and impaired male fertility.
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