Related Experiment Video
Updated: Apr 16, 2026
10:14
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Published on: May 26, 2023
4.5K
Multimodal imaging for quantifying atrophic lesions in pathologic myopia
Karolina Motloch1,2,3, Omar Moukadem1,2, Paul Goupillou1,2
1Université Paris Cité, Ophthalmology Department, AP-HP, Hôpital Lariboisière, Paris, France.
Eye (London, England)
|April 14, 2026
Summary
Infrared imaging with OCT B-scan (IR+SD-OCT) demonstrated the highest reliability and agreement for quantifying patchy atrophy in highly myopic eyes. While all modalities correlated, IR+SD-OCT showed superior consistency in measurements.
Area of Science:
- Ophthalmology
- Medical Imaging
- Retinal Diseases
Background:
- Patchy atrophy is a common complication in highly myopic (HM) eyes.
- Accurate quantification of atrophy is crucial for monitoring disease progression and treatment efficacy.
- Several imaging modalities exist, but their comparative reliability in HM eyes is not well-established.
Purpose of the Study:
- To evaluate and compare the reliability and agreement of four distinct imaging modalities for quantifying patchy atrophy in highly myopic eyes.
- To determine which imaging technique offers the most consistent measurements for clinical and research applications.
Main Methods:
- Manual delineation of atrophy area by two independent graders.
- Measurement using infrared imaging with OCT B-scan (IR+SD-OCT), blue-light fundus autofluorescence (BAF), ultra-wide-field (UWF) pseudocolor fundus photography (PCFP), and UWF green fundus autofluorescence (GAF).
- Assessment of intragrader, intergrader, and interdevice agreement using intraclass correlation coefficients (ICC) and correlation analysis.
Main Results:
- Thirty-two eyes from 30 highly myopic patients were analyzed.
- IR+SD-OCT exhibited the highest intragrader (ICC=0.998-0.999) and intergrader (ICC=0.999) repeatability.
- All modalities showed significant correlation (r=0.958-0.995, p<0.001) but differed significantly in atrophy area measurements (p<0.001).
- PCFP measured the smallest median area (2.14 mm²), while BAF measured the largest (2.90 mm²).
- Larger lesion size was associated with increased interdevice disagreement.
Conclusions:
- All evaluated imaging modalities provide reliable measurements of atrophy area in highly myopic eyes.
- Infrared imaging with OCT B-scan (IR+SD-OCT) demonstrates the strongest interdevice agreement and repeatability.
- Despite correlation, significant differences in measured atrophy areas exist between modalities, highlighting the importance of modality selection.
