Related Experiment Video
Updated: Apr 16, 2026
Author Spotlight: Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
Published on: May 26, 2023
Multimodal imaging for quantifying atrophic lesions in pathologic myopia
Karolina Motloch1,2,3, Omar Moukadem1,2, Paul Goupillou1,2
1Université Paris Cité, Ophthalmology Department, AP-HP, Hôpital Lariboisière, Paris, France.
Objectives:
To assess the reliability and agreement of four imaging modalities in quantifying patchy atrophy in highly myopic (HM) eyes.
Methods:
Atrophy area was manually delineated by two independent graders and measured using an in-built feature with four imaging modalities: infrared imaging with OCT B-scan (IR + SD-OCT), blue-light fundus autofluorescence (BAF), ultra-wide-field (UWF) pseudocolor fundus photography (PCFP) and UWF green fundus autofluorescence (GAF). Intragrader, intergrader and interdevice agreements were then assessed.
Results:
Thirty-two eyes of 30 patients were included. The mean axial length was 30.31 ± 1.56 mm. From 128 analyzed images, the best intragrader repeatability was observed with IR + SD-OCT for both graders (ICC = 0.998 and 0.999), followed by BAF (ICC = 0.998 and 0.997), UWF PCFP (ICC = 0.992 and 0.999) and UWF GAF (ICC = 0.967 and 0.985). IR + SD-OCT was also superior in terms of intergrader repeatability (ICC = 0.999), followed by ICC = 0.998 for BAF, ICC = 0.996 for UWF PCFP, and ICC = 0.985 for UWF GAF. The atrophy areas measured using each device correlated (r = 0.958-0.995; all p < 0.001), but differed significantly (p < 0.001). PCFP and BAF measured respectively the smallest and largest median area (2.14 mm² [IQR: 3.88 mm²] and 2.90 mm² [IQR: 5.28 mm²], respectively). A larger lesion size was associated with greater interdevice disagreement.
Conclusion:
All imaging modalities provided reliable atrophy area measurements, which correlated but differed significantly. IR + SD-OCT showed the strongest agreement.
