Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Proteomics approach identifies aqueous humor biomarkers in retinal diseases.

Communications medicine·2025
Same author

Proteomics approach identifies aqueous humor biomarkers in retinal diseases.

Communications medicine·2025
Same author

Evaluation of Patient-Centric Sample Collection Technologies for Pharmacokinetic Assessment of Large and Small Molecules.

Clinical pharmacology and therapeutics·2024
Same author

Overcoming Soluble Target Interference in Measurement of Total Bispecific Therapeutic Antibody Concentrations.

The AAPS journal·2023
Same author

Fc galactosylation follows consecutive reaction kinetics and enhances immunoglobulin G hexamerization for complement activation.

mAbs·2021
Same author

Characterization of a single reporter-gene potency assay for T-cell-dependent bispecific molecules.

mAbs·2019
Same journal

Updated Recommendations for the Bioanalysis of Antibody-Drug Conjugates (ADC) from the ADC working group of the AAPS Bioanalytical Community.

The AAPS journal·2026
Same journal

In vivo Predictive Dissolution Test Using Biorelevant Bicarbonate Buffer for High-dose Free Acid Drug.

The AAPS journal·2026
Same journal

Whole-Body Pharmacokinetics of Ionizable Lipid, mRNA, and the Expressed Antibody following Intravenous Administration of mRNA-Loaded Lipid Nanoparticles.

The AAPS journal·2026
Same journal

Simple Hydrodynamic Molecular Weight Model for Rapid Assessment of Therapeutic Protein Oligomerization States in Formulation.

The AAPS journal·2026
Same journal

Guiding the Molnupiravir Tablet Formulation Using Physiologically Based Biopharmaceutics Modeling and Successfully Establishing Dissolution Safe Space.

The AAPS journal·2026
Same journal

Correction: Nanotechnology-enhanced Natural Products for Cancer Chemoprevention: Molecular Mechanisms and Clinical Translation.

The AAPS journal·2026
See all related articles

Related Experiment Video

Updated: Apr 16, 2026

Field-Deployable Lens-Free Imaging Platform for Rapid Label-Free Analysis of Natural Killer Cell Activation
08:34

Field-Deployable Lens-Free Imaging Platform for Rapid Label-Free Analysis of Natural Killer Cell Activation

Published on: August 8, 2025

2.0K

Single-Eye NfL Measurement Using NULISA Technology Enables Reduction in Animal Use.

Jeongsup Shim1, Jessica Chen1, Vahan B Indjeian2

  • 1BioAnalytical Sciences, Genentech, Inc., 1 DNA Way, South San Francisco, CA, 94080, USA.

The AAPS Journal
|April 14, 2026
PubMed
Summary
This summary is machine-generated.

A new assay accurately measures neurofilament light chain (NfL) in small eye fluid samples. This reduces animal use by eightfold, enabling individual analysis for neuroaxonal damage research.

Keywords:
NULISANfLaqueous humor

More Related Videos

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
09:52

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

Published on: August 31, 2020

6.9K
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
12:18

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

Published on: December 26, 2014

22.9K

Related Experiment Videos

Last Updated: Apr 16, 2026

Field-Deployable Lens-Free Imaging Platform for Rapid Label-Free Analysis of Natural Killer Cell Activation
08:34

Field-Deployable Lens-Free Imaging Platform for Rapid Label-Free Analysis of Natural Killer Cell Activation

Published on: August 8, 2025

2.0K
Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve
09:52

Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve

Published on: August 31, 2020

6.9K
The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy
12:18

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

Published on: December 26, 2014

22.9K

Area of Science:

  • Neuroscience
  • Biomarker Discovery
  • Assay Development

Background:

  • Neurofilament light chain (NfL) is a key biomarker for neuroaxonal damage.
  • Quantifying NfL in small volumes like mouse aqueous humor (AH) traditionally requires pooling samples, increasing animal use and limiting individual data analysis.

Purpose of the Study:

  • To develop and validate a highly sensitive assay for NfL quantification in low-volume mouse AH.
  • To overcome limitations of existing immunoassays and enable individual-level data analysis.

Main Methods:

  • Developed a NfL HomeBrew (HB) assay utilizing NUcleic acid-Linked Immuno-Sandwich Assay (NULISA) technology.
  • Evaluated assay sensitivity, sample volume requirements, and performance against established platforms (Simoa, Ella).

Main Results:

  • The NfL HB NULISA demonstrated high analytical sensitivity and required minimal sample volume (<10 µL).
  • The assay reliably quantified NfL in individual mouse AH samples as low as 2 µL (90% quantifiable).
  • Performance was comparable to Simoa and superior to Ella, eliminating the need for sample pooling.

Conclusions:

  • The NULISA platform provides a sensitive and ethically advantageous method for NfL quantification in volume-restricted biological samples.
  • This assay significantly reduces animal requirements (up to eightfold) in neurobiological research.
  • Enables robust, individual-level data analysis in studies of neuroaxonal damage.