Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Biological Clocks and Seasonal Responses

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

Morphogenesis

Morphogenesis

Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.

Background and Environment Affect Phenotype

Background and Environment Affect Phenotype

Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of the Monocyte-to-High-Density Lipoprotein Ratio and the Hemoglobin-to-Red Cell Distribution Width Ratio in Post-percutaneous Coronary Intervention (PCI) Coronary Heart Disease.

Cureus·2026

Same author

Biopriming With 28-Homobrassinolide and Piriformospora indica Mitigates Cadmium Toxicity in Brassica juncea.

Physiologia plantarum·2026

Same author

Apoptosis Induction, Disruption of Cytoskeleton Remodeling and EMT Inhibition as Major Mechanism(s) Underlying the Pleiotropic Action of Withania somnifera in Breast Cancer.

Cytoskeleton (Hoboken, N.J.)·2026

Same author

Flowering Promoting Factor-Like Genes: Emerging yet Mechanistically Unresolved Regulators of Floral Transition and Plant Development.

Journal of experimental botany·2026

Same author

Anthocephalus indicus and Terminalia arjuna Combination Ameliorates Hematological and Histopathological Changes in a Rat Model of Metabolic Syndrome.

Cureus·2025

Same author

Florigen Activation Complex Dynamics and SVP-Mediated Repression Orchestrate Temperature-Regulated Flowering in Saffron.

Plant biotechnology journal·2025

Search research articles

Related Experiment Video

Updated: Apr 16, 2026

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Temperature-Responsive FPF1 Homologs Regulate Floral Transition in Saffron.

Sukriti^1,2, Joel Jose-Santhi^1,2, Diksha Kumari¹

¹Plant Adaptation and Developmental Biology Lab, Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India.

Physiologia Plantarum

|April 15, 2026

Summary

This summary is machine-generated.

Researchers identified two FLOWERING PROMOTING FACTOR 1 (FPF1)-like genes, CsatFPF1.1 and CsatFPF1.2, crucial for saffron

Keywords:

VIGS apical meristem geophytes thermoperiodic

More Related Videos

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Apr 16, 2026

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Area of Science:

Plant Molecular Biology
Agricultural Science
Genetics

Background:

Saffron (Crocus sativus L.) is a valuable crop, but its temperature-dependent flowering mechanisms are poorly understood.
Flowering in thermoperiodic plants is regulated by environmental cues, primarily temperature.
Molecular insights into saffron's floral induction are limited.

Purpose of the Study:

To identify and functionally characterize key genes regulating temperature-mediated floral induction in saffron.
To elucidate the molecular pathways controlling saffron flowering time.
To provide targets for improving saffron yield and adaptability.

Main Methods:

Gene identification and characterization of CsatFPF1.1 and CsatFPF1.2.
Analysis of gene expression patterns in saffron floral meristems.
Functional validation using ectopic expression in Arabidopsis thaliana and virus-induced gene silencing (VIGS) in saffron.

Main Results:

Two FLOWERING PROMOTING FACTOR 1 (FPF1)-like genes, CsatFPF1.1 and CsatFPF1.2, were identified as key thermoresponsive regulators.
These genes show conserved motifs, nuclear localization, and flower meristem-specific expression, upregulated under high temperatures.
Ectopic expression induced early flowering in Arabidopsis, while VIGS suppressed flowering in saffron, confirming their essential roles.
CsatFPF1.1 and CsatFPF1.2 positively regulate flowering by modulating FT gene expression in both species.

Conclusions:

CsatFPF1.1 and CsatFPF1.2 are essential positive regulators of floral initiation in saffron, mediating temperature responses.
This study provides the first in planta functional validation of flowering regulators in saffron.
The findings offer valuable genetic targets for enhancing saffron's yield and climate adaptability.