Identification of a Diagnostic Gene Signature Associated with Centrosome Amplification in Pressure Injuries: A...

Area of Science:

• Molecular biology
• Genomics
• Oncology

Background:

• Centrosome amplification (CA) is a known driver of cancer.
• CA's role in non-neoplastic conditions like pressure injuries (PI) is not well understood.
• Identifying CA-related genes (CARGs) in PI could reveal therapeutic targets.

Purpose of the Study:

• To investigate CA-related genes (CARGs) in pressure injuries (PI).
• To identify potential therapeutic targets for PI by analyzing CARGs.
• To explore the molecular mechanisms underlying CA in PI.

Main Methods:

• Transcriptomic data from 15 PI patients and 15 healthy controls were analyzed.
• Differentially expressed genes were intersected with CARGs, and key genes were identified using machine learning and ROC analysis.
• Functional enrichment, immune infiltration, drug prediction, and RT-qPCR were employed to investigate mechanisms.

Main Results:

• GADD45A, LFNG, and DUSP13 were identified as key PI-associated genes with high diagnostic performance (AUC > 0.8).
• These genes are enriched in the spliceosome pathway and correlate with neutrophil infiltration.
• Decitabine was predicted as a potential therapeutic agent, with DUSP13 showing strong binding affinity.

Conclusions:

• GADD45A, LFNG, and DUSP13 are preliminarily identified as key CA-associated genes in PI.
• Their expression patterns may support early PI identification and risk monitoring.
• Further validation in large-scale, multicenter prospective studies is required to confirm clinical translational potential.