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Updated: Apr 16, 2026
Comet Assay to Quantify DNA Damage in FLT3 Mutant-expressing 32D Cells after Exposure to Type I and Type II FLT3 Inhibitors
Published on: October 17, 2025
Reciprocal Clonal Dynamics of Independent FLT3 D835V-Positive Acute Myeloid Leukemia and Chronic Myeloid Leukemia
Kai Osone1, Seiichiro Katagiri1, SungGi Chi2
1Department of Hematology Tokyo Medical University Shinjuku Tokyo Japan.
Abstract:
We report a 65-year-old man with FLT3 D835V-mutated acute myeloid leukemia (AML) and BCR::ABL1-positive chronic myeloid leukemia (CML) that coexisted as genetically independent clones. Cytogenetic and targeted sequencing analyses demonstrated that the AML and CML clones coexisted as distinct entities. During treatment with gilteritinib, the AML blasts regressed, and then the CML clone expanded. Subsequently, the CML burden declined as the AML clone regrew. This case highlights the importance of accurately assessing clonal changes using genetic analysis when implementing molecular targeted therapy for hematologic malignancies.
