Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

2.6K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.6K
Whole Body Regeneration01:33

Whole Body Regeneration

4.7K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.7K
Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin01:26

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

1.4K
Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
1.4K
Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

6.2K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
6.2K
Skeletal Muscle Relaxants: Therapeutic Uses01:31

Skeletal Muscle Relaxants: Therapeutic Uses

1.2K
Skeletal muscle relaxants are used to relax muscle tone and alleviate painful muscle contractions. However, the choice of skeletal muscle relaxants depends on the duration of the surgical procedure in order to minimize potential side effects. Skeletal muscle relaxants like neuromuscular blocking agents [NMBAs] are commonly employed as adjuvants alongside general anesthetics in clinical settings. NMBAs are also used to maintain controlled ventilation during surgery of the larynx or pharynx...
1.2K
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

7.5K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A high-fat, high-sucrose diet exacerbates muscle and metabolic pathology and undermines glucocorticoid efficacy in dystrophin-deficient mice.

The Journal of physiology·2026
Same author

Prolonged heat stress induces autophagy in mouse skeletal muscle.

American journal of physiology. Regulatory, integrative and comparative physiology·2026
Same author

Direct aldosterone stimulation of skeletal muscle fibroblasts changes gene expression and differentially affects fibroblast functions from normal and diseased muscles.

Frontiers in physiology·2026
Same author

Spatial heterogeneity of microplastic pollution and associated emerging contaminants in tropical estuarine environments: Novel insights into distribution, bioavailability, and ecological risk.

Marine pollution bulletin·2026
Same author

A Novel Mouse Model to Identify Antigen-Specific Immune Responses in Pancreatic Cancer Cachexia.

Cancers·2026
Same author

Chronological frameworks for Indo-European languages: Insights from linguistics, archaeology and genomics.

European journal of human genetics : EJHG·2026

Related Experiment Video

Updated: Apr 16, 2026

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
07:39

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Published on: January 1, 2017

23.5K

Gasdermin E: a missing link in muscle regeneration.

Swathy Krishna, Jill A Rafael-Fortney

    The Journal of Clinical Investigation
    |April 15, 2026
    PubMed
    Summary

    Gasdermin E (GSDME) in myeloid cells is crucial for muscle regeneration after injury. Its reduced levels in aging impair this process, but IL-18 may offer therapeutic potential.

    Area of Science:

    • Muscle regeneration
    • Cellular senescence
    • Inflammation

    Background:

    • Skeletal muscle regeneration is impaired by aging and disease.
    • The microenvironment's role in muscle repair is complex.
    • Gasdermin E (GSDME) function in muscle repair is largely unknown.

    Purpose of the Study:

    • To investigate the role of GSDME in myeloid cells during skeletal muscle regeneration.
    • To determine the impact of GSDME on age-related muscle repair deficits.
    • To explore the therapeutic potential of IL-18 in aged muscle regeneration.

    Main Methods:

    • Genetic mouse models (GSDME knockout).
    • Pharmacological interventions.
    • Analysis of muscle injury, regeneration, and ectopic fat deposition.

    More Related Videos

    Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis
    09:17

    Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis

    Published on: August 24, 2017

    8.2K
    Author Spotlight: Exploring Orofacial Muscle Regeneration – Insights and Innovations
    06:33

    Author Spotlight: Exploring Orofacial Muscle Regeneration – Insights and Innovations

    Published on: December 29, 2023

    1.6K

    Related Experiment Videos

    Last Updated: Apr 16, 2026

    Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
    07:39

    Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

    Published on: January 1, 2017

    23.5K
    Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis
    09:17

    Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis

    Published on: August 24, 2017

    8.2K
    Author Spotlight: Exploring Orofacial Muscle Regeneration – Insights and Innovations
    06:33

    Author Spotlight: Exploring Orofacial Muscle Regeneration – Insights and Innovations

    Published on: December 29, 2023

    1.6K
  • Assessment of GSDME expression in young and aged human and mouse muscle.

    • Main Results:

    • GSDME in myeloid cells is essential for normal muscle regeneration after sterile injury.
    • IL-18, downstream of GSDME, prevents ectopic fat deposition in muscle.
    • GSDME expression decreases with age and is reduced after injury in old mice.
    • IL-18 partially rescues regeneration in aged GSDME-knockout mice.

    Conclusions:

    • GSDME-mediated IL-18 release is a key mechanism for effective muscle regeneration.
    • Age-related decline in GSDME contributes to impaired muscle repair.
    • Targeting the GSDME-IL-18 pathway may represent a therapeutic strategy for age-associated muscle dysfunction.