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A T-Cell Epitope Tandem Peptide Designed from Two Major Allergens of Crassostrea angulata Shows Potential for
1College of Ocean Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen, Fujian 361021, China.
Abstract:
The major allergens identified in Crassostrea angulata include tropomyosin (Cra a 1) and arginine kinase (Cra a 2). Currently, there are few studies on oral desensitization agents designed to concurrently target two oyster allergen components. In this study, a tandem peptide (Cra a T), containing T-cell epitopes of Cra a 1 and Cra a 2, was prepared based on bioinformatics and genetic engineering technology. By incubating with splenocytes from Cra a 1/Cra a 2-sensitized mouse and establishing a DC-T coculture system, it was found that Cra a T could consistently stimulate the proliferation of CD4+T cells, promote Treg cell differentiation, and upregulate the secretion of interleukin-10. Moreover, serological assays confirmed that Cra a T exhibited markedly reduced immunobinding activity against both Cra a 1 and Cra a 2. Overall, hypoallergenic Cra a T has the potential to induce dual immune tolerance and is a candidate for oyster oral desensitization preparations.
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