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Platelet-Derived Extracellular Vesicle Functionalization of Ti Implants
Published on: August 5, 2021
Cholecalciferol-loaded electrospun coating on titanium-based implant for improved osseointegration
Francisca Acevedo1, Jeyson Hermosilla2, Alexis Vera3
1Universidad de La Frontera, Faculty of Medicine, Department of Basic Sciences, Avenida Francisco Salazar 1145, 4780000 Temuco, Chile; Universidad de La Frontera, Faculty of Medicine, Center of Excellence in Translational Medicine (CEMT), and Scientific and Technological Bioresource Nucleus (BIOREN), Avenida Francisco Salazar 1145, 4780000 Temuco, Chile; Millennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, 2390123 Valparaíso, Chile.
Localized cholecalciferol delivery from nanofiber coatings accelerates bone regeneration around titanium implants. This strategy enhances early peri-implant bone formation and osseointegration without toxicity.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Orthopedic Surgery
Background:
- Aging global population increases demand for bone repair biomaterials.
- Enhancing early peri-implant bone regeneration is crucial for implant therapy success.
- Improved biological responsiveness at implant surfaces is needed.
Purpose of the Study:
- To develop cholecalciferol-loaded poly(ε-caprolactone) (PCL) nanofiber meshes for titanium mini-implants.
- To evaluate the efficacy of these coatings in promoting early peri-implant bone regeneration.
- To assess the biocompatibility and osseointegration potential of the coated implants.
Main Methods:
- Fabrication of cholecalciferol-loaded PCL nanofiber meshes via electrospinning.
- Characterization of mesh morphology, drug release kinetics, and mechanical properties.
- In vitro evaluation using mesenchymal stem cells (MSCs) and MG-63 cells for cytocompatibility and osteogenic activity.
- In vivo assessment in a rat tibia model to analyze peri-implant bone formation and osseointegration.
Main Results:
- Uniform PCL nanofiber meshes with controlled thickness and suitable mechanical properties were fabricated.
- Sustained release of cholecalciferol from the PCL matrix was achieved.
- In vitro studies confirmed cytocompatibility and enhanced osteogenic activity (ALP, collagen, mineralization).
- In vivo studies showed significantly improved early peri-implant bone volume and trabecular density around coated implants.
- No systemic or local toxicity was observed.
Conclusions:
- Localized cholecalciferol delivery via PCL nanofiber coatings effectively enhances early peri-implant bone regeneration.
- This approach accelerates implant osseointegration by modulating peri-implant biology.
- The developed biomaterial strategy offers a promising solution for improving implant therapy outcomes.
