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Updated: Apr 17, 2026

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
Published on: May 16, 2025
Re-evaluation of Helicobacter pylori diagnostic methods: A multicenter clinical diagnostic accuracy study
Bingting Yu1, Fuqin Zhao2, Xinyue Li2
1Yunnan Provincial Key Laboratory of Public Health and Biosafety, Kunming Medical University, Kunming, PR China; Department of Gastroenterology and Hepatology, Erasmus MC, Rotterdam, the Netherlands.
Objectives:
Helicobacter pylori (H. pylori) infection is the main driver of gastric cancer, with a step-wise progression via chronic superficial gastritis (CSG), atrophic gastritis (CAG), and intestinal metaplasia (IM). While accurate diagnosis of H. pylori infection is central to gastric cancer prevention strategies, current diagnostic methods may vary in sensitivity depending on the presence of gastric precursor lesions.
Methods:
In this multicenter prospective study, 462 patients undergoing upper endoscopy at three Chinese hospitals were tested using (rapid urease test [RUT], C14 urea breath test [UBT], biopsy qPCR, stool antigen test [SAT], serology, hematoxilin & Eosin [H&E] staining, and immunohistochemistry [IHC]). A composite reference defined H. pylori infection as positivity in ≥2 of UBT, SAT, or qPCR, or histological detection via H&E/IHC.
Results:
Diagnostic performance of H. pylori tests varied per gastric preneoplastic precursor lesion, with the commonly used UBT showing a low sensitivity. qPCR and SAT demonstrated superior overall performance across all stages. Combining SAT with H&E achieved the highest sensitivity (0.848 [0.765; 0.930]), while UBT + SAT offered a robust non-invasive alternative (sensitivity (0.790 [0.713; 0.866]).
Conclusion:
The accuracy of H. pylori tests varies across gastric disease stage. Multimodal diagnostic strategies are needed to improve detection and ensure accurate risk stratification in gastric cancer prevention.
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