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Updated: Apr 17, 2026

Measuring Local Anaphylaxis in Mice
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Measuring Local Anaphylaxis in Mice

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Responsible cell type in murine PAF-induced systemic anaphylaxis

Tomoyuki Suzuki1, Keisuke Yanagida2, Takao Shimizu3

  • 1Department of Lipid Life Science, National Institute of Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan.

Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids
|April 15, 2026
PubMed

Insights

Platelet-activating factor (PAF) is crucial in anaphylaxis. Targeting endothelial PAF receptors (PAFR) protects against PAF-induced mortality, suggesting endothelial cells are the main mediators.

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Platelet-activating factor (PAF) is a critical mediator in systemic anaphylaxis.
  • The specific cell type targeted by PAF during anaphylaxis is not well understood.

Purpose of the Study:

  • To determine the principal cell type targeted by PAF in systemic anaphylaxis.
  • To investigate the role of endothelial PAF receptors (PAFR) in PAF-mediated anaphylaxis.

Main Methods:

  • Utilized endothelial cell-specific PAFR-deficient mice.
  • Administered PAF to induce systemic anaphylaxis.
  • Monitored mortality rates in genetically modified and control mice.

Main Results:

  • Endothelial cell-specific PAFR-deficient mice showed significant protection from mortality.
  • This protection indicates that endothelial PAFR plays a crucial role in PAF-induced anaphylaxis.

Conclusions:

  • Endothelial PAFR is identified as the principal target mediating PAF-driven mortality in systemic anaphylaxis.
  • Targeting endothelial PAFR represents a potential therapeutic strategy for managing anaphylaxis.
Keywords:
Endothelial cellPlatelet-activating factorSystemic anaphylaxis

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