Two pattern recognition receptors provide quantitative resistance to Gray Leaf Spot in Maize

Gray Leaf Spot (GLS) caused by Cercospora zeae-maydis and Cercospora zeina is consistently among the most economically important foliar diseases in worldwide maize (Zea mays L.) production. GLS yield losses are currently controlled by genetic resistance, field management practices and foliar fungicide applications as needed, with associated costs and timing requirements; therefore, GLS resistance via a genetic solution remains an attractive option for farmers. Fine mapping, cloning and deployment of large effect resistance genes via forward breeding, genetic prediction or gene editing are viable strategies for mitigation of yield losses caused by this pathogen. This report describes the discovery, mapping, positional cloning and transgenic validation of two genes with dominant effects for resistance to Cercospora zeae-maydis (Rcz1 and Rcz2). The use of these disease resistance genes in commercial maize hybrids can provide resistance to GLS and potential reduction of yield losses in multiple markets worldwide.