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Orai1 Facilitates Angiogenesis After Myocardial Infarction Through Notch1 Signaling Pathway.

Isabel Galeano-Otero1,2,3, Beltzane Dominguez-Liste1,2, Carlos Aspron1,2

  • 1Group of Cardiovascular Pathophysiology, Institute of Biomedicine of Seville, University Hospital of Virgen del Rocio/University of Seville/CSIC, Spain (I.G.-O., B.D.-L., C.A., L.E.-I., R.D.T., A.O.-F., T.S.).

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Orai1 calcium channels are crucial for cardiac repair after myocardial infarction (MI) by promoting blood vessel growth (angiogenesis). Targeting Orai1 may offer new therapeutic strategies for heart repair following MI.

Keywords:
angiogenesiscoronary vesselsendothelial cellsinterleukin-17myocardial infarction

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Area of Science:

  • Cardiovascular Biology
  • Cellular Physiology
  • Molecular Medicine

Background:

  • Post-ischemic neovascularization is vital for cardiac repair after myocardial infarction (MI).
  • Orai1-dependent store-operated calcium entry's role in post-MI angiogenesis is not well understood.

Purpose of the Study:

  • To investigate the role of Orai1 in post-myocardial infarction (MI) angiogenesis.
  • To elucidate the mechanisms by which Orai1 influences endothelial cell (EC) function in the context of MI.

Main Methods:

  • Integrative analysis including transcriptomics, proteomics, and single-cell RNA sequencing of post-MI mouse hearts.
  • Stimulation of human umbilical vein endothelial cells (ECs) with serum from ST-segment-elevation MI patients.
  • Inhibition of Orai1 using CM4620 in zebrafish embryo models and 3D cell culture.

Main Results:

  • ST-segment-elevation MI serum upregulated VEGF, Notch, and Ca2+ signaling pathways, enhancing EC angiogenesis.
  • Orai1 expression and store-operated calcium entry were increased, crucial for EC migration and proliferation.
  • Orai1 inhibition impaired angiogenesis; Orai1 was upregulated in specific EC populations in mouse hearts and in vitro.

Conclusions:

  • Orai1 plays a significant role in post-MI angiogenesis.
  • Orai1-dependent calcium signaling is a novel mechanism supporting cardiac repair after MI.
  • Orai1 represents a potential therapeutic target for enhancing cardiac repair post-MI.