Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

19.1K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
19.1K
RNA Interference01:23

RNA Interference

29.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
29.0K
RNA Interference01:23

RNA Interference

7.9K
7.9K
Experimental RNAi02:15

Experimental RNAi

8.4K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.4K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

5.4K
5.4K
Nucleic Acid Structure01:25

Nucleic Acid Structure

10.5K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Biochimie·2026
Same author

Isoginkgetin increases the expression of metal responsive transcripts but inhibits their translation.

PloS one·2026
Same author

Arbuscular mycorrhiza provides postanthesis benefits to maximize wheat grain yield and nitrogen concentration.

The New phytologist·2026
Same author

Aptamers: Current Applications in Leukemia Diagnostics and Therapeutics.

Nucleic acid therapeutics·2026
Same author

Rethinking yield stability through phenotypic plasticity and its link to modern statistical methods.

Journal of experimental botany·2026
Same author

Torpor-Induced Regulation of Poly(A) Tail Machinery in 13-Lined Ground Squirrel Brown Adipose Tissue.

Journal of developmental biology·2026
Same journal

Stable, micron-scale lipocondensates achieving prolonged circulation without PEGlyated lipids.

Biomaterials science·2026
Same journal

Fiber and continuum scale contributions to the intrinsic and apparent fracture of soft collagenous tissue <i>via</i> cutting.

Biomaterials science·2026
Same journal

Surface morphology-regulated tissue adhesion in solid and mesoporous silica-reinforced gelatin nanocomposite hydrogels.

Biomaterials science·2026
Same journal

Nanostructured hyaluronic acid-chia mucilage film as bioactive wound dressings for accelerated skin regeneration.

Biomaterials science·2026
Same journal

Tunable bio-inspired hybrid hydrogels reprogram stem cell-derived extracellular vesicles for superior wound regeneration.

Biomaterials science·2026
Same journal

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026
See all related articles

Related Experiment Video

Updated: Apr 18, 2026

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

8.4K

Supramolecular RNAi with multifunctional siRNA nanostructures.

Michael Shaikhet1, Joshua O'Grady2, Filiz K Collak2,3

  • 1Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, K1S 5B6 8, Canada.

Biomaterials Science
|April 16, 2026
PubMed
Summary
This summary is machine-generated.

Researchers created novel RNA nanostructures for targeted cancer gene therapy. These structures precisely silence oncogenes, showing significant anti-cancer effects in cell studies, paving the way for new treatments.

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.1K
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K

Related Experiment Videos

Last Updated: Apr 18, 2026

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

8.4K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

8.1K
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

11.6K

Area of Science:

  • Biotechnology
  • Nanomedicine
  • RNA Therapeutics

Background:

  • Supramolecular RNA nanostructures are emerging as advanced genetic tools in biomedicine.
  • Genetically encoded short interfering RNA (siRNA) nanostructures offer precise oncogene silencing for cancer gene therapy.

Purpose of the Study:

  • To design and synthesize V- and Y-shaped branched RNA templates for assembling 2D and 3D siRNA nanostructures.
  • To target Glucose Regulated Proteins (GRP75, 78, 94, 170) mRNA transcripts in cancer cells.
  • To investigate the anti-cancer activity of these novel nanostructures.

Main Methods:

  • Synthesis of V- and Y-shaped branched RNA templates.
  • Scaffolding of self-assembled 2D and 3D siRNA nanostructures (nano-squares, -cubes, -tubes).
  • Structure and stability analyses, bioconjugation with fluorescent reporters (fluorescein, coumarin) for FRET-based monitoring.
  • In vitro studies using human adenocarcinoma (A549) cell line.

Main Results:

  • Formation of well-defined siRNA nano-squares, -cubes, and -tubes with specific supramolecular and biophysical properties.
  • Successful incorporation of fluorescent reporters for real-time monitoring of nanostructure assembly.
  • Demonstrated cell uptake and intracellular localization of siRNA nanostructures in A549 cells.
  • Significant anti-proliferative and toxicity effects observed, indicating anti-cancer activity via GRP silencing.

Conclusions:

  • The study highlights the innovation of multifunctional siRNA nanostructures for cancer gene-silencing therapies.
  • These nanostructures show potential for precise targeting and effective elimination of cancer cells.
  • Further research is warranted to explore their therapeutic applications in oncology.