Stress-induced proteome remodeling at the Golgi-endosome interface

Area of Science:

• Cell Biology
• Molecular Biology
• Cancer Research

Background:

• Cellular stress responses are crucial for survival and often exploited by cancer.
• Understanding Golgi apparatus (Golgi) stress responses is vital for cancer insights.
• The role of Golgi stress in cancer remains incompletely understood.

Purpose of the Study:

• To investigate cellular responses to Golgi stress.
• To identify stress-induced changes at the Golgi apparatus.
• To explore the role of Golgi stress in cancer, particularly melanoma.

Main Methods:

• Utilized small molecule stress models.
• Performed proteomic analyses to identify stress-induced changes.
• Investigated the expression and localization of FAM129A/NIBAN1.

Main Results:

• Depletion of Golgi transport proteins is a common response to various Golgi stressors.
• Ionophores induce de novo expression of FAM129A/NIBAN1, which localizes to the secretory pathway.
• Constitutive FAM129A expression and relocalization occur in melanoma cells with an EMT-like phenotype.

Conclusions:

• The Golgi-endosome interface is a critical hub for cellular responses to Golgi stress.
• Cancer cells, especially melanoma, exhibit specific responses to Golgi stress involving FAM129A.
• These findings offer insights into the role of Golgi stress in cancer biology and progression.