Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molnupiravir Treatment Associated With Clearance of Cache Valley Virus From Cerebrospinal Fluid in an Immunocompromised Child With Meningoencephalitis.

Open forum infectious diseases·2026
Same author

The impact of clinical metagenomic testing on patient management: facts versus fantasy.

The Lancet. Infectious diseases·2026
Same author

Probable Japanese Encephalitis Virus Transmission through Organ Transplantation.

The New England journal of medicine·2026
Same author

Next-Generation Sequencing Reveals Continued Circulation of Rare HIV-1 Subtypes in the Democratic Republic of the Congo and Refines the Estimate of the Emergence Dates of Three Subtypes.

Viruses·2026
Same author

Implementation and outcomes of a rapid response genomic hospital epidemiology programme at an academic medical centre over 7 years.

The Lancet. Microbe·2026
Same author

<i>Trichosporon inkin</i> meningitis in a pediatric patient diagnosed via metagenomic sequencing.

ASM case reports·2026

Related Experiment Video

Updated: Apr 18, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
11:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Published on: December 27, 2010

12.9K

Development and genomic characterization of a diverse HIV-1 variant reference panel for nucleic acid-based testing.

Jiangqin Zhao1, Tianxiong Li1, Hanxia Huang1

  • 1Laboratory of Molecular Virology, DETTD, OBRR, CBER, FDA, 10903 New Hampshire Ave, Silver Spring, MD, United States.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|April 16, 2026
PubMed
Summary

A diverse HIV-1 reference panel was created to address challenges in HIV nucleic acid testing (NAT) due to global genetic diversity. This panel aids in evaluating the analytical performance of HIV NAT assays.

Keywords:
NATPCRdiagnosticsgenetic diversityhuman immunodeficiency virusp24recombinantsubtypeviral load

More Related Videos

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

19.5K
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

12.9K

Related Experiment Videos

Last Updated: Apr 18, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
11:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Published on: December 27, 2010

12.9K
An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings
19:57

An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Published on: March 30, 2014

19.5K
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

12.9K

Area of Science:

  • Virology
  • Molecular Biology
  • Public Health

Background:

  • Global Human Immunodeficiency Virus (HIV) genetic diversity complicates accurate detection and diagnosis via nucleic acid testing (NAT).
  • Well-characterized reference panels are crucial for assessing the analytical performance of HIV diagnostic tests.

Purpose of the Study:

  • To develop a comprehensive reference panel for HIV NAT that accurately represents the genetic diversity of circulating HIV strains.
  • To create a standardized tool for validating HIV detection methods.

Main Methods:

  • HIV-1 strains were cultured from clinical specimens in Cameroon.
  • Near full-length genomes were sequenced using metagenomic next-generation sequencing and Sanger sequencing.
  • A diverse subset of 18 HIV-1 strains was selected to form the reference panel, with viral loads quantified at low, medium, and high levels.

Main Results:

  • Near full-length HIV-1 genomes (average 9589 bp) were obtained for 37 out of 59 strains.
  • The panel includes 18 diverse HIV-1 strains: 4 subtypes, 4 circulating recombinant forms, and 8 unique recombinant forms.
  • Panel members were prepared at three viral load concentrations (low, medium, high), resulting in 51 total panel members.

Conclusions:

  • The developed HIV reference panel effectively represents diverse circulating strains.
  • This panel is a valuable resource for evaluating the performance of HIV NAT assays.
  • The panel is available to developers and manufacturers of HIV diagnostic tests upon request.