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CrMo6 based POMOF-nanozyme for colorimetric and electrochemical dual-mode precise sensing dopamine
Yunjie Li1, Yawen Guan1, Xinyu Han1
1Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Key Laboratory of Molecular Cytogenetics and Genetic Breeding, College of Life Science and Technology, Harbin Normal University, Harbin 150025, Heilongjiang Province, China.
A novel dual-mode biosensor accurately detects dopamine (DA), a key biomarker for neurological disorders. This platform offers portable, precise sensing in various samples, advancing disease diagnostics and food safety.
Area of Science:
- Biomarker Detection
- Nanomaterials
- Biosensors
Background:
- Dopamine (DA) is a critical biomarker for neurodegenerative and psychiatric disorders.
- Accurate and efficient DA sensing is essential for disease diagnostics but remains challenging.
- Existing methods for DA detection often lack portability and precision.
Purpose of the Study:
- To develop a dual-mode sensing platform for precise and portable detection of dopamine (DA).
- To utilize a novel peroxidase-like nanoreactor for enhanced sensing capabilities.
- To validate the platform's performance in complex biological and food matrices.
Main Methods:
- Construction of a dual-mode (colorimetric and electrochemical) sensing platform using a peroxidase (POD)-like nanoreactor, CuBTC@CrMo6.
- Cascade sensing of hydrogen peroxide (H2O2) and DA in colorimetric mode.
- Electrochemical detection of DA over a broad concentration range.
- Validation of the sensor in serum and milk samples.
Main Results:
- The colorimetric mode achieved LODs of 10.13 μM for H2O2 and 0.51 μM for DA.
- The electrochemical mode offered a detection range of 0.1-150 μM with an ultra-low LOD of 31.03 nM for DA.
- Satisfactory recoveries (98.6%-106.2%) and low relative standard deviation (RSD < 4%) were obtained in serum and milk.
- Mechanistic studies confirmed the critical role of POD-like activity in self-correcting detection.
Conclusions:
- The developed POMOF-based nanoreactors provide robust, multimodal biosensors for biomarker detection.
- This dual-mode platform offers significant advancements for both clinical diagnostics and food safety applications.
- The self-correcting detection mechanism enhances the reliability of the biosensor.
