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Updated: Apr 18, 2026

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
A copper substitution approach for the selective analysis of chlorophyll-derived porphyrins in modern geological and
Ruochen Yang1, Xiaowei Xia1, Junyang Chen1
1State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing 102249, China.
Abstract:
The input of chlorophyll provides key information for reconstructing paleoproductivity and tracing diagenesis in geological matrices. However, accurate analysis is hampered as chlorophyll readily suffers from demetallation and carbon-chain cleavage, leading to a complex mixture of modified Mg(II) porphyrins and porphins. Here, we developed a copper substitution pretreatment that selectively converts native chlorophyll, its derived Mg(II) porphyrins and porphins (chlorophyll-derived porphyrins) into stable Cu(II) porphyrins, thus enabling a comprehensive qualitative and quantitative analysis of the chlorophyll-derived pool. The method was evaluated using model compounds and ultra-performance liquid chromatography coupled with ultra-high-resolution mass spectrometry (UPLCOrbitrap MS). It demonstrated >95% conversion of chlorophyll a with CuCl2 and 89% for porphin with Cu sheets, whereas non-target Ni(II), V(IV), and Fe(III) porphyrins remained unaffected. Applied to lake sediments, the pretreatment enabled specific analysis of chlorophyll-derived porphyrins against complex geological matrix. This strategy unifies chlorophyll-derived porphyrins into a single detectable form, offering a selective approach for the accurate reconstruction of initial chlorophyll input and composition in geological samples.
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