Validation of a quantitative analysis method for tryptophan metabolites in asthmatic mice and bacteria using GC-MS/MS

This study established a gas chromatography-tandem mass spectrometry (GC-MS/MS) method for the simultaneous quantification of 25 tryptophan (TRP) metabolites encompassing the kynurenine pathway, serotonin pathway, and indole pathway. The method was systematically validated in terms of precision and accuracy, demonstrating robust reliability. Application of this method to bacterial cultures as well as serum and lung tissues from asthmatic mice revealed significant alterations in TRP metabolic profiles. Owing to its high throughput and sensitivity, this method is well suited for the analysis of TRP metabolites in complex biological matrices and provides a robust platform for comprehensive profiling of TRP metabolites in complex biological matrices, facilitating studies of TRP metabolism in asthma.