PcabHLH58/PcabHLH151 regulates adventitious root development and nitrogen uptake in poplar
Hantian Wei1,2, Xinglu Zhou1,2, Xiaodong Xiang1
1State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Northeast Forestry University, Beijing, China.
Nature Communications
|April 16, 2026
Summary
Two key genes, PcabHLH58 and PcabHLH151, are crucial for adventitious root (AR) development and nitrogen uptake in Populus. Their regulatory network provides insights for poplar genetic breeding.
Area of Science:
- Plant Biology
- Genetics
- Ecology
Background:
- Populus species are vital for ecological shelterbelts and timber in China.
- Adventitious root (AR) formation and root architecture are key for Populus adaptability and propagation.
- Understanding the genetic basis of root development is crucial for improving Populus.
Purpose of the Study:
- Identify genes regulating adventitious root (AR) development in Populus cathayana.
- Elucidate the regulatory network controlling AR formation and nitrogen uptake.
- Provide insights for genetic breeding of Populus.
Main Methods:
- Genome-wide association studies (GWAS) to identify candidate genes.
- Gene mutation analysis to assess functional roles.
- Gene expression and pathway analysis to understand regulatory mechanisms.
Main Results:
- PcabHLH58 and PcabHLH151 were identified as key genes for AR development and nitrogen uptake.
- Mutations in these genes significantly inhibited AR formation and nitrogen assimilation.
- A regulatory network involving PcaSCL33, PcaPLT1, PcaWOX5, and PcaPAR1 was elucidated.
Conclusions:
- PcabHLH58 and PcabHLH151 play critical roles in poplar adventitious root development.
- A novel gene regulatory network controlling root architecture and function in Populus has been uncovered.
- This study offers a foundation for enhancing Populus traits through genetic breeding.
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