Related Experiment Video
Updated: Apr 18, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Chromosome level genome assembly of the American sloughgrass (Beckmannia syzigachne)
Wei Tang1, Chuanlin Yin2, Huangjie Gao3
1State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311401, China.
Abstract:
American sloughgrass [Beckmannia syzigachne (Steud.) Fernald] is a problematic annual grass weed in winter wheat fields of China, which causes great loss of wheat yield. A lack of high-quality genome resources has hindered understanding of the Herbicide resistance characteristics and ecological adaptations. Here, we combined Illumina short read, PacBio long-read, and high-throughput chromosome conformation capture (Hi-C) sequencing technologies to generate a high-quality, chromosome-scale genome assembly of B. syzigachne. The genome assembly was 3.19 Gb in size, consisting of seven pseudo-chromosomes. The contig and scaffold N50 values were 62.2 Mb and 431.7 Mb, respectively. The genome assembly completeness was estimated at 97.1% by BUSCO assessment. Annotation revealed 36,944 protein-coding genes and 88.83% repeat sequences. This high-quality genome assembly is a valuable resource for future fundamental research and agricultural management of B. syzigachne, and provides significant new insights into the herbicide resistance as well as the adaptive evolution of B. syzigachne.
Related Concept Videos
Genome Annotation and Assembly
Karyotyping

