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Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
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Volumetric Scattering Microscopy.

Zijun Gao, Keyi Han, Zhi Ling

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    Volumetric scattering microscopy (VSM) offers a new way to see inside biological tissues. This innovative optical-computational method enables clear 3D fluorescence imaging by using scattered light as an encoding resource.

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    Area of Science:

    • Biomedical optics
    • Microscopy
    • Biophotonics

    Background:

    • Optical scattering in biological tissues hinders fluorescence imaging, limiting 3D visualization.
    • Current methods for imaging through scattering media are often complex or lack axial precision.

    Purpose of the Study:

    • To develop a practical framework for 3D fluorescence imaging in scattering biological media.
    • To overcome the limitations of existing techniques in terms of instrumental complexity and axial ambiguity.

    Main Methods:

    • Introduced Volumetric Scattering Microscopy (VSM), a scan-free, optical-computational approach.
    • Utilized an aperture-segmented Fourier light-field configuration to capture angularly resolved speckle-encoded fluorescence.
    • Employed adaptive feature-based descattering and joint sub-pupil alignment for volumetric reconstruction.

    Main Results:

    • Achieved high-fidelity 3D fluorescence imaging in phantoms, cell systems, ex vivo tissues, and intact Xenopus embryos.
    • Demonstrated preserved spatial resolution, enhanced optical sectioning, and quantitative accuracy under strong scattering.
    • Showcased robust, large-field volumetric imaging capabilities in diverse scattering environments.

    Conclusions:

    • VSM transforms scattered light into a structured encoding resource for 3D imaging.
    • This framework simplifies 3D fluorescence imaging in complex biological systems.
    • VSM provides a scalable pathway for routine volumetric imaging through scattering tissues.