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Researchers developed a device for light sheet fluorescence microscopy (LSFM) to image samples at an air-liquid interface (ALI). This innovation expands LSFM applications to biological specimens previously incompatible with the technique.

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Area of Science:

  • Biomedical Engineering
  • Microscopy Techniques
  • Cell Biology

Background:

  • Light sheet fluorescence microscopy (LSFM) offers gentle, high-speed volumetric imaging.
  • Conventional LSFM systems have limited sample chambers, preventing studies at air-liquid interfaces (ALI).

Purpose of the Study:

  • To develop a device enabling robust ALI imaging using LSFM.
  • To expand the utility of LSFM for previously inaccessible biological models.

Main Methods:

  • Designed and implemented a novel device for ALI imaging on an upright LSFM with dipping objectives.
  • Demonstrated system utility with diverse biological samples, including mouse salivary glands, human epidermal equivalents, and Drosophila brains.

Main Results:

  • Successfully enabled robust imaging of biological specimens at an air-liquid interface using LSFM.
  • Validated the system's versatility across ex vivo, in vitro, and in vivo models.

Conclusions:

  • The developed device significantly broadens the applicability of LSFM.
  • This adaptable device blueprint can facilitate LSFM adoption in new research areas requiring ALI conditions.