Related Experiment Video

Updated: Jul 15, 2026

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
08:00

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

Published on: October 14, 2016

Defective mononuclear phagocyte function in patients with myelomonocytic leukemia and in some patients with lymphoma

M J Cline

    The Journal of Clinical Investigation
    |September 1, 1973
    PubMed

    Insights

    Mononuclear phagocytes in myelomonocytic leukemia and lymphoma patients show impaired bacterial killing. This defect may contribute to frequent and antibiotic-resistant infections in these patients.

    Area of Science:

    • Immunology
    • Hematology
    • Infectious Diseases

    Background:

    • Mononuclear phagocytes are crucial for host defense against bacterial infections.
    • Patients with myeloproliferative and lymphoproliferative diseases often experience recurrent infections.

    Purpose of the Study:

    • To investigate the microbicidal function of mononuclear phagocytes in patients with myelomonocytic leukemia and lymphoma.
    • To determine if impaired phagocyte function contributes to infection susceptibility in these patient groups.

    Main Methods:

    • Mononuclear phagocytes were isolated from patients with myelomonocytic leukemia and lymphoma.
    • Phagocytosis and intracellular bacterial replication were assessed for several bacterial species.
    • The effect of antibiotics on intracellular bacteria was evaluated.

    Main Results:

    • Mononuclear phagocytes from 8/10 myelomonocytic leukemia patients and 2/9 lymphoma patients phagocytized bacteria.
    • These phagocytes failed to inhibit intracellular bacterial replication normally.
    • Intracellular bacteria were protected from antibiotics present in the medium.

    Conclusions:

    • A defect in the microbicidal function of malignant monocytes is present in some patients with myelomonocytic leukemia and lymphoma.
    • This impaired function may partially explain the high incidence of infections and antibiotic-resistant infections in these patients.

    More Related Videos

    Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro10:05

    Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

    Published on: January 25, 2017

    Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma10:52

    Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

    Published on: March 30, 2018

    Related Concept Videos

    Differentiation of Common Myeloid Progenitor Cells01:15

    Differentiation of Common Myeloid Progenitor Cells

    Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
    Classification of Leukocytes01:30

    Classification of Leukocytes

    Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
    Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
    Disorders of Leukocytes01:27

    Disorders of Leukocytes

    Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
    Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
    Cells of the Innate Immune Response01:28

    Cells of the Innate Immune Response

    The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
    Phagocytes
    Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
    Immune Surveillance by NK Cells and Phagocytes01:25

    Immune Surveillance by NK Cells and Phagocytes

    Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
    Natural Killer Cells: The Fast Responders
    NK cells are large granular lymphocytes found in the blood and lymphatic system. These...