Establishment of an Agrobacterium-Mediated Transformation System for Blastospores of Naematelia aurantialba

Over the past few decades, Naematelia aurantialba (commonly known as "Mushroom Jin Er") has attracted significant attention due to its medicinal properties and nutritional value. To address the existing gap in genetic manipulation tools for molecular biology research in N. aurantialba, we employed Agrobacterium tumefaciens-mediated transformation to introduce exogenous genes into the blastospores of N. aurantialba for the first time. We successfully established a genetic transformation method for N. aurantialba. Moreover, this work not only shows that N. aurantialba blastospores are an excellent recipient material for future molecular studies but also provides strong technical support and a valuable reference for accelerating genetic research related to N. aurantialba. Key methodology points include: Establishment of an Agrobacterium-mediated transformation system for the blastospores of N. aurantialba for the first time. Transformants with hygromycin B resistance were screened, and molecular identification results confirmed that the exogenous gene had been successfully introduced into the genome of blastospores. Phenotypic analysis showed that the blastospores of transformants had GUS activity and green fluorescence signals, confirming that the gus gene and gfp gene could be expressed in the transformants.