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Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
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In-Solution Characterization of Extracellular Vesicles: A New Approach to Evaluating Antibody Binding and Surface
Maria-Anthi Kakavoulia1, Peter Spies2, Tanja J Kutzner3
1Biochemistry and Chemistry, Department of Veterinary Sciences, Ludwig-Maximilians-Universität Munich, München, Germany.
Journal of Extracellular Vesicles
|April 17, 2026
Summary
Flow-Induced Dispersion Analysis (FIDA) quantifies extracellular vesicle (EV) interactions with antibodies, offering a high-resolution method for analyzing surface epitopes and molecular binding kinetics without immobilization.
Area of Science:
- Biotechnology
- Nanotechnology
- Immunology
Background:
- Extracellular vesicles (EVs) show promise as diagnostic and therapeutic tools.
- Analyzing nanoscale EVs and their molecular interactions is challenging.
- Quantitative single-vesicle analysis of surface epitopes is limited.
Purpose of the Study:
- To apply Flow-Induced Dispersion Analysis (FIDA) for characterizing extracellular vesicle (EV)-antibody interactions.
- To evaluate FIDA's ability to measure EV size and interaction kinetics.
- To assess epitope accessibility and antibody binding on different EV sources.
Main Methods:
- Utilized Flow-Induced Dispersion Analysis (FIDA), a microfluidic technique.
- Measured hydrodynamic size and interaction kinetics using fluorescent ligands.
- Characterized EV-antibody interactions using anti-CD63 antibodies on engineered and primary EVs.
Main Results:
- FIDA measured EV diameters between 40-90 nm, differing from Nanoparticle Tracking Analysis (NTA) results (70-200 nm).
- Calculated CD63-binding EC50 values revealed higher epitope abundance on engineered EVs.
- Maximal antibody loading occurred at 0.5-5 nM, with varying percentages for different antibodies.
Conclusions:
- FIDA is a high-resolution, label-efficient tool for quantifying EV-antibody interactions.
- FIDA enables precise measurement of epitope accessibility on EVs.
- FIDA can support EV standardization and quality control workflows.
