Nano-bio interface modulation using Garcinia spicata-capped silver nanoparticles (GS-AgNPs) for combating...

Area of Science:

• Nanotechnology
• Green Chemistry
• Materials Science

Background:

• Silver nanoparticles (AgNPs) possess diverse biological activities.
• Phyto-synthesis offers a sustainable and eco-friendly approach for nanoparticle production.
• Garcinia spicata (GS) is a plant with potential phytochemical constituents suitable for biosynthesis.

Purpose of the Study:

• To synthesize silver nanoparticles (AgNPs) using Garcinia spicata (GS) leaf extract.
• To characterize the synthesized GS-AgNPs using various analytical techniques.
• To evaluate the antimicrobial and anti-inflammatory potential of the synthesized GS-AgNPs.

Main Methods:

• Biosynthesis of AgNPs using GS leaf extract.
• Characterization using UV-Vis spectroscopy, XRD, FTIR, and FESEM.
• Antimicrobial activity testing against MRSA and Pseudomonas aeruginosa.
• In vitro anti-inflammatory assays including protein denaturation, membrane stabilization, and nitric oxide scavenging.

Main Results:

• UV-Vis spectroscopy confirmed AgNPs synthesis via surface Plasmon resonance at 420 nm.
• FESEM analysis revealed spherical GS-AgNPs with diameters ranging from 30-60 nm.
• Significant antimicrobial activity was observed against MRSA and Pseudomonas aeruginosa at 12 µg/mL.
• GS-AgNPs demonstrated notable inhibition of inflammatory mediators in various assays.

Conclusions:

• Garcinia spicata leaf extract is an effective and sustainable source for green synthesis of AgNPs.
• The synthesized GS-AgNPs exhibit promising antimicrobial and anti-inflammatory properties.
• This study represents the first report on the green synthesis of GS-AgNPs using Garcinia spicata.