Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A FRET-Mediated AIE Biosensor Based on Functionalized Peptide-Stabilized Gold Nanoclusters for Sensitive Detection of Matrix Metalloproteinase-2.

Analytical chemistry·2026
Same author

<i>De novo</i> design of NIR-II thioxanthene dye and phosphate-driven charge transfer-coupled <i>J</i>-aggregates for high resolution tumor angiography and type I phototherapy against hypoxic tumors.

Chemical science·2026
Same author

Ion-Exchange-Mediated Enhancement of Lactate Oxidase Delivery for Breast Tumor Imaging and Therapy.

Analytical chemistry·2026
Same author

One-Pot Ligation-Recombinase Polymerase Amplification-Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a-Powered Trimode Lateral Flow Assay for Sensitive MicroRNA Detection.

Analytical chemistry·2026
Same author

Mitochondria-Targeted Fluorescent Probe for Super-Resolution Imaging of HNO in Lung Cancer.

Analytical chemistry·2026
Same author

Triple-Response Peptide Probe for Simultaneous Monitoring the Activity of Three Proteases through a Single Nanopore.

Analytical chemistry·2026

Related Experiment Video

Updated: Apr 19, 2026

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.9K

Dual-Lock NIR Dual-Channel Probe: Synchronous Viscosity/HSO3- Sensing for Visualizing Platinum-Induced Nephrotoxicity

Siyu Gao1, Lulu Zhang1, Wenwen Li2

  • 1College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.

Analytical Chemistry
|April 17, 2026
PubMed
Summary

New fluorescent probes help assess platinum drug kidney toxicity and test protective agents, aiding safer cancer chemotherapy.

More Related Videos

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.4K
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Published on: March 9, 2021

3.9K

Related Experiment Videos

Last Updated: Apr 19, 2026

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.9K
Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney
10:00

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

Published on: October 12, 2015

12.4K
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
10:25

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

Published on: March 9, 2021

3.9K

Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Molecular Imaging

Background:

  • Platinum-based chemotherapy agents, while effective, cause significant nephrotoxicity, limiting their use.
  • Acute kidney injury (AKI) is a major concern in patients receiving cisplatin (DDP).
  • Simultaneous monitoring of cellular microenvironment viscosity and bisulfite (HSO3-) concentration is crucial for understanding DDP-induced AKI.

Purpose of the Study:

  • To design and validate novel near-infrared dual-channel fluorescent probes (BTXPI and BTXVI) for simultaneous detection of viscosity and HSO3-.
  • To investigate the limitations of confocal microscopy versus super-resolution microscopy in subcellular analysis.
  • To evaluate the nephrotoxicity of different platinum-based drugs and assess renoprotective agents.

Main Methods:

  • Development of benzothiazole-derived fluorescent probes with a "dual-lock" mechanism.
  • Utilizing confocal microscopy and super-resolution structured illumination microscopy (SIM) for cellular imaging.
  • In vivo studies in murine models to assess drug toxicity and renoprotective efficacy.

Main Results:

  • The dual-channel probes successfully quantified viscosity and HSO3- concentration, key biomarkers for DDP-induced AKI.
  • Super-resolution microscopy revealed limitations of confocal imaging in resolving subcellular colocalization.
  • Cisplatin was identified as the most nephrotoxic platinum drug; renoprotective agents combined with DDP alleviated kidney injury without reducing antitumor effects.

Conclusions:

  • The developed "dual-lock" fluorescent probes are effective tools for monitoring complex biological parameters and assessing drug toxicity.
  • These probes facilitate the optimization of combination therapies for safer and more effective cancer chemotherapy.
  • The study highlights the importance of advanced imaging techniques for accurate subcellular analysis.