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Oncogenic tRF-24-O7M8LO9LIM drives lung adenocarcinoma progression by disrupting the CD82/p53 tumor suppressor

Sinan Liu1, Kui Zhu2, Yixuan Wu1

  • 1Department of Laboratory Medicine, Nantong First People's Hospital and The Second Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong 226001, China.

Biochemical Pharmacology
|April 17, 2026
PubMed

Insights

Transfer RNA-derived small RNAs (tsRNAs) like tRF-24-O7M8LO9LIM promote lung adenocarcinoma (LUAD) progression. This tsRNA targets CD82 to regulate the p53 pathway, impacting tumor growth and patient prognosis.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Transfer RNA-derived small RNAs (tsRNAs) are emerging regulators in cancer.
  • The role of tsRNAs, specifically tRF-24-O7M8LO9LIM, in lung adenocarcinoma (LUAD) is largely unknown.

Purpose of the Study:

  • To investigate the molecular mechanisms and biological functions of tRF-24-O7M8LO9LIM in LUAD.
  • To determine the prognostic significance and therapeutic potential of tRF-24-O7M8LO9LIM in LUAD.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for expression analysis.
  • In vitro (cell lines) and in vivo (nude mice) assays for functional validation.
  • Dual-luciferase assay, KEGG pathway analysis, and western blotting for mechanism elucidation.

Main Results:

  • tRF-24-O7M8LO9LIM is upregulated in LUAD tissues and cells, correlating with advanced TNM stage and tumor size.
  • Knockdown of tRF-24-O7M8LO9LIM inhibits LUAD cell proliferation, migration, invasion, and tumor growth.
  • tRF-24-O7M8LO9LIM directly targets CD82, regulating the p53 signaling pathway.

Conclusions:

  • tRF-24-O7M8LO9LIM acts as an oncogenic tsRNA in LUAD.
  • Targeting tRF-24-O7M8LO9LIM or its downstream CD82/p53 pathway may offer therapeutic strategies for LUAD.
Keywords:
CD82Lung adenocarcinomaTumor progressionp53 signaling pathwaytRF-24-O7M8LO9LIM

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