Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Analyzers: Common Types

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A multi-dimensional approach combining HPLC, ion mobility and high resolution mass spectrometry for investigating transition metal complexes.

Scientific reports·2026

Same author

Evaluation of recombinant trypsinogen and chymotrypsinogen production in Komagataella phaffii through co-expression of HAC1 and PDI1.

Microbial cell factories·2026

Same author

Non-agricultural feedstocks for next-generation biomanufacturing with yeasts.

Current opinion in biotechnology·2026

Same author

Bioconversion of the C1 substrate methanol to succinic acid with engineered Komagataella phaffii.

Bioresource technology·2026

Same author

Characterization of black oat root exudates in the presence of interspecific weed species and intraspecific neighbors, and their effects on root traits.

Frontiers in plant science·2026

Same author

Rewiring methanol metabolism in Komagataella phaffii through implementation and evolution of a heterologous RuMP cycle.

Metabolic engineering·2026

Search research articles

Related Experiment Video

Updated: Apr 19, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Rapid quantitative bioanalysis using LC-HRIM-QTOFMS with flexible sample delivery and variable traveling wave

Sabrina M Cramer¹, Viktoria Kowarz², Diethard Mattanovich²

¹BOKU University, Institute of Analytical Chemistry, Department of Natural Sciences and Sustainable Resources, Muthgasse 18, 1190, Vienna, Austria.

Analytica Chimica Acta

|April 17, 2026

Summary

This summary is machine-generated.

This study introduces rapid quantification methods for small molecules in complex biological samples using high-resolution ion mobility (HRIM) and optimized sample delivery. These advancements enable faster, more sensitive bioanalytical LC-MS applications with improved data quality.

Keywords:

Biotechnology High-throughput Ion mobility Phytosiderophores Riboflavin Structures for lossless ion manipulation

More Related Videos

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Related Experiment Videos

Last Updated: Apr 19, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

Area of Science:

Analytical Chemistry
Biochemistry
Mass Spectrometry

Background:

High-resolution ion mobility (HRIM) enhances bioanalytical LC-MS sensitivity and throughput.
Conventional HRIM methods face limitations in data points per peak and working range, hindering quantification.
This research addresses these limitations for improved small molecule and metabolite analysis.

Purpose of the Study:

To develop rapid, quantitative bioanalytical methods using HRIM.
To overcome the trade-offs between HRIM separation speed and data quality.
To establish high-throughput workflows for complex biological samples.

Main Methods:

Developed new HRIM-QTOFMS workflows for rapid quantification.
Utilized variable traveling wave profiles to increase LC peak sampling (>15 points/peak).
Optimized LC sample introduction with feed injection and short LC columns (<5 min/sample).

Main Results:

Achieved rapid quantification of riboflavin and flavin nucleotides in yeast samples (<5 min/sample).
Successfully identified and quantified phytosiderophores in plant samples (<3 min/sample).
Demonstrated agreement with validated LC-MS/MS methods for phytosiderophore quantification.

Conclusions:

Presents a novel combination of rapid LC, HRIM, and feed injection for quantitative bioanalysis.
HRIM traveling wave profiles enhance LC peak sampling for high-throughput analysis.
Enables routine analysis of small molecules in biological samples, including isomer quantification.