Related Experiment Video For 17-hydroxyprogesterone

Updated: Apr 21, 2026

A Novel Method: Super-selective Adrenal Venous Sampling

A Novel Method: Super-selective Adrenal Venous Sampling

Published on: September 15, 2017

Is a systematic adrenocorticotropic hormone (ACTH) stimulation test useful in diagnosing nonclassical congenital

Bénédicte Ferreira¹, Geoffroy Robin¹, Pascal Pigny²

¹Unité fonctionnelle de Gynécologie Endocrinienne, Service de Gynécologie Médicale, Orthogénie et Sexologie, Hôpital Jeanne de Flandre, Centre Hospitalier Universitaire de Lille, Lille, France.

Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology

|April 18, 2026

Abstract:

The ACTH stimulation test is the gold standard for diagnosing NCAH. However, this test has a human and material cost. While some teams perform it as a second-line test, others use it as a first-line test to avoid false negatives associated with single basal 17-hydroxyprogesterone (17-OHP) serum levels. In this prospective, single-center, cross-sectional observational study, we included 210 women aged from 15 to 41 who were referred for oligoanovulation and/or hyperandrogenism from November 2022 to June 2024. In this population, we determined the proportion of false negatives for NCAH using basal 17-OHP serum levels by performing ACTH stimulation tests. Among the 210 patients included, no NCAH was diagnosed, and no false negatives of basal 17-OHP serum levels were identified. Basal 17-OHP serum levels were significantly higher in PCOS patients but remained below the 2 ng/ml threshold and were significantly correlated with hormonal and ultrasound markers of PCOS. Consequently, the systematic use of the ACTH stimulation test in women with hyperandrogenism and/or oligoanovulation does not appear to be a cost-effective diagnostic strategy.

Keywords:

17-hydroxyprogesterone 21-hydroxylase deficiency ACTH stimulation test nonclassical congenital adrenal hyperplasia polycystic ovary syndrome

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Keywords:

17-hydroxyprogesterone 21-hydroxylase deficiency ACTH stimulation test nonclassical congenital adrenal hyperplasia polycystic ovary syndrome

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