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Related Experiment Video

Updated: Apr 20, 2026

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

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Spatially Controlled Capture and Site-Resolved Analysis of Single Extracellular Vesicles.

Joong Bum Lee1, Donato Conteduca1, Mi Ho Jeong1

  • 1Center For Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|April 18, 2026
PubMed
Summary

This study introduces a new nanowell array method for precise extracellular vesicle (EV) detection. The technique ensures accurate single-EV fluorescence measurements, improving analysis of these important biomarkers.

Keywords:
extracellular vesiclesfluorescence microscopynanoparticlesnanowell arraysquantitative imaging

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Last Updated: Apr 20, 2026

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Quantitative fluorescence analysis of single extracellular vesicles (EVs) is challenged by non-uniform particle distribution on surfaces.
  • Accurate signal counting is obscured by heterogeneous particle loading, complicating EV characterization.

Purpose of the Study:

  • To develop a simple, array-based method for unambiguous, site-specific fluorescence measurements of single EVs.
  • To improve the accuracy and reliability of extracellular vesicle detection and analysis.

Main Methods:

  • Coupling site-specific capture into nanowells with mask-gated image analysis for precise EV localization.
  • Utilizing a PDMS translation step to clear excess particles, ensuring single EV capture in discrete nanowells.
  • Registering in-well signals to a bright-field nanowell mask for accurate fluorescence detection.

Main Results:

  • Achieved >99% EV capture efficiency at designated locations on nanowell arrays.
  • Enabled accurate detection of EV fluorescence signals with significantly reduced background noise.
  • Demonstrated accurate tracking of programmed EV mixture ratios and HER2 positivity in breast cancer EVs.

Conclusions:

  • Established a reliable pathway for array-based extracellular vesicle detection using nanowell arrays.
  • The method facilitates precise manipulation and analysis of EVs and other nanoparticles.
  • The technique is adaptable for plasma-derived EVs, multi-channel fluorescence imaging, and mixed cargo analysis.