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Updated: Apr 21, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
Recent advances in mapping protein networks regulated by post-translational modifications via proximity tagging
1Skaggs Graduate School of Chemical and Biological Sciences, Scripps Research, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA; Department of Chemistry, Scripps Research, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.
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Recent platform innovations are now enabling the resolution of the different ways protein-protein interactions (PPIs) are wired by post-translational modifications (PTMs). Such PTM-aware interactome maps are facilitating the systematic cataloguing of context-dependent network changes. This review highlights developments from 2023 to 2025, focusing on acetylation, methylation, phosphorylation, ubiquitination, redox-regulated cysteine networks, and glycosylation, and outlines analytical and biological frontiers. Together, these advancements are shifting PTM-informed proximal interactome mapping toward mechanism-grounded, actionable frameworks.
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