Related Experiment Video
Updated: Apr 21, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
Resistant starch type 3 prepared with Corynebacterium glutamicum glycogen debranching enzyme and its application in
Abbas Ismail1, Sulukana Banglamat2, Watthanachai Saradhuldhat2
1Center of Excellence in Structural and Computational Biology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
None:
This study has evaluated the effect of glycogen debranching enzyme from Corynebacterium glutamicum (CgGDE) on the production of resistant starch type 3 (RS3) from tapioca starch. Treatment of 10% gelatinized starch with 1 unit of CgGDE/g starch at 25 °C for 3 h followed by retrogradation for 24 h at 4 °C yielded modified starch containing 27.5% RS3, a sixfold increase in resistant starch (RS) compared with native starch and 2.3-fold higher RS content than obtained after pullulanase treatment. CgGDE increased the proportion of long amylopectin chains (degree of polymerization ≥13), facilitating the formation of retrograded structures with increased thermal stability and sharp B-type crystallinity. Despite the lower specific volume and firmer texture of RS3-fortified white bread, the partial substitution (≤20%) of wheat flour with modified starch increased RS content and slightly decreased glycemic index. These results demonstrate the potential of CgGDE for the preparation of RS3 and its subsequent food-related applications.
More Related Videos
05:22Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Published on: January 25, 2022
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Related Concept Videos
Production of Organic Acids
Microbes in Food Production
Microbes in the Production of Fermented Foods
Bioreactor Controls-III
Fungal Group Zygomycota
Introduction to Carbohydrates