Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of magnesium supplementation on inflammatory factors and clinical outcomes of allogeneic hematopoietic stem cell transplantation in patients with acute leukemia receiving a neutropenic diet: a randomized clinical trial.

Scientific reports·2026
Same author

CRISPR/Cas9-mediated knockout of TopBP1 shifts the Bax/Bcl-2 balance toward apoptosis in MCF7 breast cancer cells.

Molecular biology reports·2026
Same author

Human small non-coding tRNA-derived fragments as key hallmarks of host-virus interaction in viral infections.

Cell & bioscience·2026
Same author

Molecular epidemiology and phylogenetic analysis of Anaplasma ovis and Anaplasma marginale in Ixodidae infesting livestock in northwestern Iran.

BMC veterinary research·2026
Same author

Patterns of dietary habits are associated with metabolic health status in adults.

The British journal of nutrition·2026
Same author

Novel Insights into Pontocerebellar Hypoplasia Type 3: Discovery of a New Disease-causing PCLO Variant and Development of a CRISPR-generated Cell Model : Novel Insights into Pontocerebellar Hypoplasia Type 3.

Galen medical journal·2026

Related Experiment Video

Updated: Jun 30, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.9K

A Comprehensive Review on CRISPR-Based Screening and Its Applications.

Ali Saber Sichani1, Mahmoud Hassani2, Fatemeh Gila3

  • 1Department of Biology, Texas A&M University, College Station, TX, 77843, USA.

Molecular Biotechnology
|April 19, 2026
PubMed
Summary

CRISPR screens are powerful tools for studying gene function in cells. This review covers CRISPR library design, delivery, and selection methods for various applications like drug response and cancer research.

Keywords:
Arrayed screeningCRISPR screeningCRISPR/Cas9High-throughput sequencingPooled screening

More Related Videos

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.7K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.5K

Related Experiment Videos

Last Updated: Jun 30, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.9K
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
09:05

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

23.7K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

1.5K

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • CRISPR-based tools are now standard in biology and medicine.
  • CRISPR screens are essential for large-scale loss-of-function and perturbation studies.

Purpose of the Study:

  • To review the use of pooled CRISPR screens for interrogating gene function in living cells.
  • To contrast pooled and arrayed CRISPR screening formats.
  • To discuss recent applications and future directions of CRISPR screening.

Main Methods:

  • Focus on pooled CRISPR screens analyzing cell fitness or selectable markers.
  • Contrast with arrayed formats offering richer molecular readouts like transcriptome-wide changes.
  • Discuss library design, delivery, and selection strategies using various Cas nucleases (Cas9, Cas12, Cas13).

Main Results:

  • Different Cas nucleases expand the scope of genome and transcriptome perturbations that can be studied.
  • CRISPR screens have diverse applications in drug response, viral infection, and cancer biology.
  • Advancements in high-content technologies and data analysis are enhancing screening capabilities.

Conclusions:

  • CRISPR screening is a versatile technology for gene function analysis.
  • Improvements are driving the next generation of CRISPR-based screening studies.
  • Emerging diagnostic uses highlight the expanding role of CRISPR technology.