A Mechanical Robust Tunable Terepthaloyl Modified Chitosan Hydrogel Matrix for Modulating Biological Response.

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Chitosan hydrogels offer biocompatibility and antibacterial properties for tissue engineering.
• Chemical crosslinking can enhance chitosan hydrogel properties.
• Developing advanced materials is crucial for effective tissue regeneration.

Purpose of the Study:

• To develop a novel crosslinked chitosan hydrogel using terephthaloyl chloride.
• To evaluate the antibacterial efficacy and biocompatibility of the developed hydrogel.
• To assess the hydrogel's potential in promoting cell proliferation and angiogenesis for tissue engineering.

Main Methods:

• Chitosan hydrogels were chemically crosslinked with terephthaloyl chloride at varying concentrations (0.25, 0.5, 0.75, 1 mmol).
• Antibacterial activity was tested against Escherichia coli and Staphylococcus aureus.
• Cell proliferation and cytotoxicity were assessed using fibroblast cells.
• Vascular endothelial growth factor expression was analyzed.

Main Results:

• Terephthaloyl chloride-crosslinked chitosan (TPC-CS) hydrogels showed enhanced antibacterial effects compared to pure chitosan (CS).
• The TPC-CS4 formulation (1 mmol) significantly promoted epithelial cell proliferation.
• TPC-CS4 stimulated vascular endothelial growth factor expression, indicating increased angiogenic potential.
• Cytotoxicity experiments confirmed the excellent biocompatibility of TPC-CS4 hydrogels.

Conclusions:

• Chemical crosslinking of chitosan hydrogels with terephthaloyl chloride improves structural integrity and antibacterial properties.
• The TPC-CS4 hydrogel demonstrates significant potential for promoting cell proliferation and angiogenesis.
• Terephthaloyl chloride-based chitosan hydrogels are promising biomaterials for diverse tissue engineering applications.