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Structure and Function of Platelets01:18

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The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
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Related Experiment Video

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Purification of Platelets from Mouse Blood
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Lyophilized Synthetic Platelets: In Vitro Characterization and In Vivo Evaluation in Mouse Thrombocytopenia Model.

Ujjal Didar Singh Sekhon1, Dante Disharoon2, Shrijal S Desai1

  • 1Haima Therapeutics LLC, Cleveland, OH, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|April 20, 2026
PubMed
Summary

Researchers developed a shelf-stable lyophilized synthetic platelet (Lyo-SP) to overcome limitations of current transfusions. This innovation offers a readily available, effective alternative for treating bleeding complications.

Keywords:
hemorrhagehemostasislyophilized synthetic plateletplatelet transfusionplatelets

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Hematology

Background:

  • Current platelet transfusions rely on donor platelets with short shelf-lives (5-7 days) due to bacterial contamination risks.
  • Existing alternatives like cold-stored or freeze-dried platelets still face donor-dependency challenges.
  • Liposome-based synthetic platelets (SP) were previously developed but exhibit storage instability in aqueous suspension.

Purpose of the Study:

  • To develop a lyophilized, shelf-stable synthetic platelet (Lyo-SP) for improved availability and on-demand use.
  • To evaluate the physico-chemical stability, hemostatic function, biosafety, and in vivo efficacy of Lyo-SP.

Main Methods:

  • Synthetic platelets (SP) were lyophilized using lyoprotectants to create Lyo-SP.
  • Lyo-SP stability was assessed through morphology, size, and charge analysis under various storage conditions.
  • Platelet-mimetic functions were evaluated using multiparametric assays with human plasma and blood.
  • Biosafety was determined by analyzing Lyo-SP's effects on endothelial cells, neutrophils, RBCs, and complement C3.
  • In vivo efficacy was tested in a tail-clip bleeding model in thrombocytopenic mice.

Main Results:

  • Lyo-SP maintained consistent morphology, size, and charge during long-term storage at various temperatures.
  • Lyo-SP effectively conserved platelet-mimetic functions in human plasma and blood.
  • Lyo-SP demonstrated biosafety, showing no adverse effects on key blood components and endothelial cells.
  • Lyo-SP significantly reduced bleeding in a mouse model of thrombocytopenic bleeding.

Conclusions:

  • Lyophilization successfully created a shelf-stable synthetic platelet (Lyo-SP).
  • Lyo-SP retains essential characteristics and functions for hemostasis.
  • Lyo-SP presents a promising, safe, and effective alternative to donor platelets for managing bleeding disorders.