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Characterization of a novel Mycobacterium tuberculosis serine protease Rv1815 in regulating bacterial metabolism and

Qishun Feng1, Qiao Lin2, Zhang Xia1

  • 1National Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen 518112, China.

Current Research in Microbial Sciences
|April 20, 2026
PubMed

Insights

A novel serine protease, Rv1815, from Mycobacterium tuberculosis (Mtb) enhances bacterial virulence and intracellular survival. This protease also impacts Mtb

Area of Science:

  • Microbiology
  • Molecular Biology
  • Pathogenesis

Background:

  • Serine proteases are key virulence factors in Mycobacterium tuberculosis (Mtb).
  • Understanding the specific roles of individual proteases is crucial for developing novel therapeutic strategies against tuberculosis.

Purpose of the Study:

  • To characterize the function and impact of the novel serine protease Rv1815 in Mtb.
  • To investigate Rv1815's role in Mtb pathogenesis, intracellular survival, and antibiotic resistance.

Main Methods:

  • Enzyme activity assays to confirm serine protease function of Rv1815.
  • Proteomic analysis to identify Rv1815-regulated proteins.
  • Macrophage infection models to assess intracellular survival and growth.
  • In vitro growth assays and morphological analysis.
  • Antibiotic resistance assays, specifically for rifampicin.

Main Results:

  • Rv1815 exhibits serine protease activity and is secreted extracellularly, localizing within macrophage cytoplasm.
  • Rv1815 is essential for virulence, survival, metabolism, and antibiotic resistance protein expression in Mtb.
  • Rv1815 promotes Mtb intracellular survival in macrophages, enhances in vitro growth, and alters bacterial morphology.
  • Rv1815 negatively regulates Mtb resistance to rifampicin.

Conclusions:

  • Rv1815 is a significant contributor to Mtb physiology and virulence.
  • This serine protease plays a critical role in host-pathogen interactions and Mtb pathogenesis.
  • Rv1815 represents a potential novel target for anti-tuberculosis therapies.
Keywords:
Antibiotic resistanceMacrophage survivalMetabolismMycobacterium tuberculosisSerine protease

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