Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Lingo01:11

Genetic Lingo

83.8K
Overview
83.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Weight misperception among deaf and hard-of-hearing adults in the United States: Results from NHANES, 1999-2018.

Disability and health journal·2026
Same author

Role of ATP Hydrolysis and Product Release in the Translocation Mechanism of SARS-CoV-2 NSP13.

The journal of physical chemistry. B·2024
Same author

The Role of ATP Hydrolysis and Product Release in the Translocation Mechanism of SARS-CoV-2 NSP13.

bioRxiv : the preprint server for biology·2023
Same author

Ni<sup>2+</sup> and Cu<sup>2+</sup> complexes of N-(2,6-dichlorophenyl)-N-mesityl formamidine dithiocarbamate structural and functional properties as CYP3A4 potential substrates.

Scientific reports·2023
Same author

Computational studies of potential antiviral compounds from some selected Nigerian medicinal plants against SARS-CoV-2 proteins.

Informatics in medicine unlocked·2023
Same author

Allostery Inhibition of BACE1 by Psychotic and Meroterpenoid Drugs in Alzheimer's Disease Therapy.

Molecules (Basel, Switzerland)·2022

Related Experiment Video

Updated: Apr 22, 2026

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English
14:34

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

247

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English.

Blessed P Mbogo1, Samantha R Fernsebner2, Monsurat M Lawal1

  • 1Drug Discovery Lab, School of Science, Technology, Accessibility, Mathematics, and Public Health (STAMP), Gallaudet University.

Journal of Visualized Experiments : Jove
|April 20, 2026
PubMed
Summary

This study identifies potential natural compounds to selectively inhibit Polo-like Kinase 1 (PLK1) in breast cancer by targeting its polo-box domain (PBD). The computational approach aims to minimize off-target effects on related proteins.

More Related Videos

Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
07:15

Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation

Published on: January 16, 2019

10.2K
Portable Intermodal Preferential Looking IPL: Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism
10:11

Portable Intermodal Preferential Looking IPL: Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism

Published on: December 14, 2012

22.1K

Related Experiment Videos

Last Updated: Apr 22, 2026

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English
14:34

A Bilingual Computational Workflow for Identifying Potential PLK1 Inhibitors in American Sign Language and English

Published on: April 3, 2026

247
Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
07:15

Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation

Published on: January 16, 2019

10.2K
Portable Intermodal Preferential Looking IPL: Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism
10:11

Portable Intermodal Preferential Looking IPL: Investigating Language Comprehension in Typically Developing Toddlers and Young Children with Autism

Published on: December 14, 2012

22.1K

Area of Science:

  • Computational drug discovery
  • Molecular biology
  • Oncology

Background:

  • Polo-like Kinase 1 (PLK1) is crucial for cell cycle progression and is overexpressed in cancers like breast cancer.
  • Targeting PLK1's polo-box domain (PBD) offers a selective strategy to induce apoptosis in cancer cells.
  • High structural similarity between PLK1 and its homologs (PLK2, PLK3) necessitates highly selective inhibitors to avoid toxicity.

Purpose of the Study:

  • To develop a computational workflow for identifying selective PLK1-PBD inhibitors from natural products.
  • To prioritize drug candidates with potential anti-cancer activity against breast cancer.
  • To ensure selectivity against homologous proteins (PLK2, PLK3) crucial for normal physiological functions.

Main Methods:

  • Utilized a bilingual computational workflow integrating virtual screening, structural clustering, and protein-ligand docking.
  • Analyzed interactions with PLK1-, PLK2-, and PLK3-PBD structures using the SuperNatural 3.0 database.
  • Performed ADMET-S profiling and quantum mechanical (QM) stability analysis for prioritized compounds.

Main Results:

  • Filtered natural products based on breast cancer relevance and drug-likeness criteria.
  • Identified and clustered diverse chemical structures for interaction analysis.
  • Generated hypotheses for selective PLK1-PBD inhibition, prioritizing natural product candidates.

Conclusions:

  • The computational workflow successfully generated testable hypotheses for selective PLK1 inhibition.
  • Prioritized natural-product-derived compounds warrant further investigation through experimental validation.
  • This approach aids in the development of targeted cancer therapies with reduced off-target effects.