Nolandella shirvanensis sp. nov. (Amoebozoa, Tubulinea) and species diversity within the genus Nolandella
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Updated: Apr 22, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Alexander Kudryavtsev1, Ekaterina Piven1, Maria Palataicova1
1Laboratory of Cellular and Molecular Protistology, Zoological Institute of the Russian Academy of Sciences, Universitetskaya nab. 1, 199034 Saint-Petersburg, Russia.
Abstract:
The paper describes Nolandella shirvanensis sp. nov., a newly identified species of the marine amoeba genus Nolandella Page, 1983 (Tubulinea, Nolandida). It represents the first record of this genus from an athalassohaline biotope: a cold mud volcano with methane seeps in Gobustan National Park (Azerbaijan). Amoebae were isolated and cultivated in seawater with a salinity of 30‰, but they had an experimentally determined salinity tolerance range of 10-120‰. Morphological and molecular phylogenetic analyses show that the genus Nolandella comprises three named species (i.e., N. hibernica (Page, 1980), N. abertawensis (Page, 1980), and N. shirvanensis sp. nov.) and a fourth species, distinct from other members of this genus, known from the strain Nolandella sp. ATCC 50913. Molecular analysis of the diversity of Nolandella spp. expands the number of known actin paralogs in this genus and demonstrates a unique insertion in the mitochondrial cytochrome c oxidase subunit 1 (Cox1) gene, with a 590 bp group I intron nested inside it. This is the second detection of a group I intron in the Cox1 gene in a member of the Tubulinea.
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